儿童急性白血病Bcl-2基因MDR1基因表达及意义(英文)

Expression of Bcl-2 and MDR_1 Genes in Children with Acute Leukemia

目的 研究Bcl 2 ,MDR1基因与儿童急性白血病耐药的关系及其临床意义。方法 采用逆转录多聚酶链式反应 (RT PCR)技术 ,对 36例急性白血病患儿及 10例血小板减少性紫癜患儿 (对照 )骨髓单个核细胞中Bcl 2和MDR1基因的表达进行检测。结果 ①初治组、复发组Bcl 2基因表达明显高于对照组 ,差异均有显著性(P <0 .0 5 )。初治组、完全缓解组Bcl 2基因表达明显低于复发组 ,差异均有显著性 (P <0 .0 1)。复发组MDR1基因的表达明显高于对照组、初治组、完全缓解组 (P <0 .0 1或 0 .0 5 )。初治组与对照组间及完全缓解组与对照组间的MDR1表达差异无显著性 (P >0 .0 5 )。②Bcl 2和MDR1基因的表达与白血病临床特征如性别、年龄、初诊时白细胞数、骨髓中幼稚细胞百分数及肝、脾、淋巴结肿大程度均无显著相关性 (P >0 .0 5 )。③Bcl 2与MDR1基因之间无显著相关性 (rs=0 .30 8,P>0 .0 5 )。结论 Bcl 2和MDR1基因可能通过不同的机制导致白血病的耐药。

Objective To investigate the expression and significance of Bcl 2 and MDR 1 genes in children with acute leukemia. Methods The expressions of Bcl 2 and MDR 1 genes in 36 cases of acute leukemia and 10 cases of idiopathic thrombocytopenic purpura in the control group were examined by RT PCR. Results The expressions of Bcl 2 in the incipient group and relapsed group were higher than that of the control group (P< 0.05 ). While in the incipient group and complete remission group, they were lower than that of the relapsed group (P< 0.01 ). The expression of MDR 1 gene in the relapsed group was higher than those of control group, incipient group and complete remission group (P< 0.01 or 0.05 ). There was no difference of MDR 1 expression between the incipient group and the control group or the complete remission group and the control group (P> 0.05 ). There was no close relationship between the levels of Bcl 2 or MDR 1 and clinical features such as gender, age, initial WBC count, the percent of carcinocytes, hepatomegaly, splenomegaly and lymphadenhypertrophy (P> 0.05 ). The relationship between Bcl 2 and MDR 1 genes was not significant (r s= 0.308 , P> 0.05 ). Conclusions Bcl 2 and MDR 1 genes were associated with drug resistance by different mechanisms.