窒息新生儿血浆非蛋白结合铁的测定及意义

Determination of Plasma Nonprotein-Bound Iron in Newborns with Asphyxia

目的 探讨非蛋白结合铁在新生儿窒息后再灌注损伤中的作用。方法 用克服基体效应的Bleomycin方法 ,分别测定 2 0例窒息新生儿生后 6h内、6～ 12h和 12～ 72h的血浆非蛋白结合铁 (NPBI) ,并取 2 0例正常新生儿于出生 6h内测定其血浆非蛋白结合铁作为对照。结果 窒息组NPBI阳性率明显高于对照组(80 %vs 2 0 % ) ,P <0 .0 1。窒息组血浆非蛋白结合铁水平在生后 6h内为 [(4 .14± 2 .4 1) μmol/L]、6～ 12h为(2 .2 6± 2 .2 1) μmol/L ,均比对照组 [(0 .2 8± 0 .79) μmol/L]显著升高 (P <0 .0 5 ) ,尤以生后 6h内为最高。结论 非蛋白结合铁可能在新生儿窒息后再灌注损伤中起着重要的作用。

Objective To study the effect of nonprotein bound iron (NPBI) on neonatal postasphyxial reperfusion injury. Methods Plasma concentrations of NPBI from 20 asphyxiated newborns were measured serially with the matrix effect free bleomycin assay during 0 to 6 hours, 6 to 12 hours and 12 to 72 hours after birth. They were then compared with those from 20 healthy newborns within 6 hours after birth (controls). Results The positive NPBI rate was significantly higher in asphyxiated newborns than that in the controls (80% vs 20%). NPBI was significantly elevated in asphyxiated newborns during 0 to 6 hours [( 4.14 ± 2.41 ) μmol/L] and 6 to 12 hours [( 2.26 ± 2.21 ) μmol/L] compared with that in the controls [( 0.28 ± 0.79 ) μmol/L] (P< 0.05 ). The highest level of NPBI was noted during 0 to 6 hours after birth. Conclusions NPBI may play an important role in neonatal postasphyxial reperfusion injury.