血管紧张素Ⅱ对人脐静脉内皮细胞单核细胞趋化因子-1表达的影响

Effect of angiotensin Ⅱ on expression of monocyte chemoattractant protein-1 in cultured human umbilical vein endothelial cells

目的 探讨血管紧张素Ⅱ (AngⅡ )对人脐静脉内皮细胞 (HUVECs)分泌单核细胞趋化因子 1(MCP 1)的影响。方法 用RT PCR和ELISA方法检测AngⅡ在不用时间和浓度以及氯沙坦干预后对HUVECs分泌MCP 1的影响。结果 AngⅡ显著刺激HUVECs表达MCP 1,MCP 1mRNA分别在AngⅡ的浓度为 1× 10 - 7mol/L和刺激 4h时表达最强烈 ;而MCP 1蛋白质分泌随刺激时间延长而增加 ;氯沙坦干预则明显减低MCP 1的表达。结论 AngⅡ可强烈刺激HUVECs表达MCP 1。

Objectives To evaluate the effect of angiotensin Ⅱ on the protein and mRNA expression of monocyte chemoattractant protein 1(MCP 1) in cultured human umbilical vein endothelial cells(HUVECs) Methods The levels of protein and mRNA of MCP 1 were detected by ELISA and RT PCR respectively. Results Angiotensin Ⅱ distinctly increased the expression of MCP 1 in HUVECs; The highest expression was performed at 1×10 -7 mol/L angiotensin Ⅱ or at the 4th hour respectively; and losartan reduced the expression of MCP 1 remarkably. Conclusions Angiotensin Ⅱ can significantly increase the expression of MCP 1 in HUVECs,which is antagonized by losartan.