CD10在儿童期B-急性淋巴细胞白血病中的表达特点及其在微小残留病检测中的意义

Characterization of CD10 Expression and Its Significance in Minimal Residual Disease Detection in Childhood B-Acute Lymphoblastic Leukemia

本研究的目的是观察CD10在儿童期B 急性淋巴细胞白血病 (B ALL)中的表达情况 ,并探讨CD10在白血病微小残留病检测中的应用价值。用流式细胞术分析 5 8例儿童期急性淋巴细胞白血病表型 ,观察CD10在B ALL病例骨髓细胞中的表达特点 ,并将CD10抗体与其它多种抗体构成四色组合用于B ALL的微小残留病检测 ,评价其所起到的作用。结果表明 ,6 5 .5 % (38/5 8)的B ALL具有CD10过强表达 (CD10 bright)的特点 ,而且CD10 bright与CD34的阳性表达率有关———CD10 bright多出现于CD34阳性率高的B ALL中。CD10 bright在微小残留病检测中 ,与其它异常表达的抗原形成的组合能够在双参数点图上很好地区分出白血病细胞群与正常细胞群。即使在其他抗原表达无异常时 ,也可因CD10的过强表达而使白血病细胞群与正常细胞群区分开。结论 :CD10 bright与B ALL中CD34的高比例表达相关 ,CD10 bright也是微小残留病检测的一个很好的指标 ,CD10的表达与其他用于微小残留病检测的抗原表达的应用有互补作用。

To observe the expressions of CD10 in childhood B acute lymphoblastic leukemia (B ALL) and to define the role of CD10 in minimal residual disease (MRD) detection. 58 cases of childhood B ALL were studied in this program. Four color flow cytometry was used to analyze the characteristics of B ALL phenotypes. The four color fluorochrome labeled antibody combinations of CD10 with other markers were used to detect MRD. The results showed that CD10 overexpression (CD10 bright ) was detected in 65.5% (38/58) of B ALL patients and a strong correlation between CD10 bright and CD34 expression was also observed, i.e. CD10 bright expression most frequently happened in B ALL with high percentage of CD34 positive cells. In detection of MRD, CD10 bright , combined with other markers, could effectively distinguish normal cells with leukemic cells, even if there was no any other marker that can be used. It is concluded that CD10 bright expression correlates with high expression of CD34 in B ALL, it is a good marker for MRD detection. The combination of CD10 and other markers can be applied in B ALL MRD detection with flow cytometry.