rhG-CSF和rhSCF对正常猴外周血白细胞和CFU-GM数的影响

The Effects of rhG-CSF and rhSCF on Peripheral Blood Leukocytes and CFU-GM in Rhesus Monkeys

为了观察重组人干细胞因子 (rhSCF)和 (或 )粒细胞集落刺激因子 (rhG CSF)动员外周血干细胞的效果 ,本研究将 15只正常成年猕猴分成 3组 ,每天 1次 ,连续 14天分别皮下注射赋形剂、rhG CSF 10 μg/ (kg·d)和rhSCF5 0 μ/ (kg·d) +rhG CSF 10 μ/ (kg·d)。结果表明 ,单独给rhG CSF组的外周血白细胞数最高值在给药后第 7天出现 ,为给药前值的 4 11% ,其后很快下降。联合给药组外周血白细胞数最高值在第 9天出现 ,为给药前值的 5 38% ,第 7至第 9天的外周血白细胞数在 5 35 % - 5 38%之间。单独给药组外周血CFU GM最高值在给药后第 5天为给药前值的 937% ,联合给药组在第 9天为 1175 %。结论 :rhG CSF对外周血干 /祖细胞有明显的动员作用 ,rhSCF +rhG CSF联合动员的效果优于rhG

To evaluate the effects of rhG CSF and rhSCF on mobilization of the peripheral blood stem cells, 15 monkeys were divided into control, rhG CSF 10 μg/(kg·day) and rhG CSF 10 μg/(kg·day)+rhSCF 50 μg/(kg·day) treated groups. Monkeys were administered with vehicle, rhG CSF and rhG CSF+rhSCF subcutaneously once daily for 14 days, respectively. The results showed that the highest counts of leukocyte of rhG CSF treated group were 411% of baseline value on day 7 after administration, compared with that of rhG CSF+rhSCF treated group which were 538% on day 9. The highest counts of leukocytes lasted for 3 days in combined treated group. CFU GM from peripheral blood in the two groups were 8.37 and 11.75 times higher at 5 and 9 days respectively after the mobilization. It is concluded that rhG CSF significantly increases the number of peripheral blood leukocytes and CFU GM, and a better effect can be obtained by rhSCF+rhG CSF combined administration.