低分化急性髓系白血病细胞的形态学及免疫表型特征

The Morphological and Immunological Characteristics of Less-Differen-tiated Acute Myeloid Leukemic Cells

本研究的目的是探讨低分化急性髓系白血病 (AML Mo)的细胞形态学、组织化学、免疫学特征及其诊断意义。用瑞氏 姬姆萨混合染色法及组织化学试剂对骨髓涂片进行染色分类 ,并采用流式细胞术作细胞免疫表型分析。结果表明 ,2例患者均符合AML Mo分类诊断标准 ,髓过氧化物酶 (POX)染色阳性率均为阴性 ,糖原 (PAS)染色均为细颗粒阳性 ,髓系单抗CD33、CD13阳性 ,T淋巴系单抗CD2、CD3阴性 ,B淋巴系单抗CD10、CD19、CD2 2阴性。结论 :骨髓细胞形态学 ,组织化学及免疫分型是诊断AML Mo的主要依据 ,采用多种单克隆抗体同时染色血细胞及进行多参数分析 ,有利于检测多种抗原在单个细胞上的表达 ,使AML Mo的诊断更加准确。

The aim was to study the morphological, histochemical and immunological characteristics of less differentiated acute myeloid leukemic cells, and their diagnostic significance. Wrigt Giemsa and histochemical staining were used to stain bone marrow smears from 2 case of AML Mo. Immunological phenotypes were determined with flow cytometry. The results showed that myeloperoxidase stainings of both cases were negative, PAS was positive with fine particles, CD33/CD13 were positive, CD2/CD3/CD10/CD19/CD22 were negative. It is concluded that morphology, histochemistry and immunological phenotype on bone marrow smears are the main diagnostic basis for AML Mo. The use of multiple monoclonal antibodies for staining may improve the accuracy.