脐血中CD34^+CD38^+细胞含量对急性白血病患者移植后造血重建的影响

Effect of the Number of CD34^+CD38^+ Cells in Cord Blood on Hematopoietic Reconstitution in Patients with Acute Leukemia after umbilical Cord Blood Transplantation

为了探讨非亲缘脐血移植中CD34+ CD38+ 细胞回输量对造血重建的影响 ,采用流式细胞术分析复苏后的CD34+ CD38+ 细胞数 ,并对 2 0例急性白血病患者的体重、中性粒细胞 (ANC)和血小板 (Plt)恢复时间进行测定。结果表明 :2 0例接受的CD34+ CD38+ 细胞输入量为 (9.85 - 32 5 .71)× 10 4/kg ,其ANC恢复 >5× 10 8/L的中位时间为 18 5 (11- 32 )天。在 19例患者中Plt恢复 >2× 10 10 /L的中位时间为 4 5 (12 - 118)天。CD34+ CD38+ 细胞输入量与ANC和Plt恢复时间存在相关 ,r值分别为 - 0 .5 77(P <0 .0 1)和 - 0 .5 0 3(P <0 0 5 )。结论 :CD34+ CD38+细胞含量与造血恢复时间相关 ,足量输入CD34+ CD38+

The objective of this research was to explore whether the number of CD34 +CD38 + cells infused affects hematopoietic reconstitution after cord blood transplantation. The number of CD34 +CD38 + cells in cord blood was analysed with flow cytometry after freezethawing. The body weight and time for neutrophil and platelet recovery were measured in 20 children with acute leukemia. The results showed that the median number of CD34 +CD38 + cells infused was 29.47(9.85-325 71)×10 4/kg. A median time for neutrophil recovery (>5×10 8/L) in 20 patients was 18.5(11-32) days, and time for platlet recovery (>2×10 10 /L) in 19 of 20 patients was 45(12-118) days. The number of CD34 +CD38 + cells infused correlated with time to neutrophil and platelet recovery (r=-0.577, P<0.01 and r=0 503, P<0.05, respectively). In conclusion, the number of CD34 +CD38 + cells infused is correlated with the time for hematologic recovery.