乙烯雌酚诱发大鼠泌乳素腺瘤动物模型的建立

Development of a prolactin pituitary tumor model in Wistar rats induced by diethylstilbestrol

目的建立大鼠泌乳素腺瘤动物模型,观察大鼠不同性别、用药时间和去势等因素对其发生的影响。方法将Wistar大鼠分为注射乙烯雌酚(DES)的雌性鼠试验组、去势鼠试验组和雄性鼠试验组,每组内设对照组。分别于第2,4,8和12周时观察大鼠脑垂体重量及血清泌乳素(PRL)水平和垂体组织病理学改变。结果雌性组和去势组8周时大鼠均形成腺瘤,血清PRL水平达高峰。雄性组12周时大鼠均形成腺瘤,PRL水平和垂体重量随着DES作用时间的延长而持续增高。结论腹腔注射DES可以引起Wistar大鼠泌乳素腺瘤的形成,是一种制作简便、可靠的动物模型,大鼠性别、是否去势对其形成无明显影响。

Objective To develop a prolactinoma model in Wistar rats and to investigate the influence of the gender differences, time taking of the diethylstilbestrol (DES) and ovariectomy. Methods Wistar rats were divided into DES-treated female group (F), DES-treated ovariectomized female group (O), DES-treated male group (M) and their control groups. Five rats in DES-treated and control groups were executed respectively after 2, 4, 8, and 12 weeks to investigate the weight of pituitary gland, serum prolactin (PRL) level, pituitary light microscope and immunocytochemistry. Results By 8 weeks, 100% pituitary glands of F and O developed prolactinomas, associated with increased serum PRL levels, peaked at 8 weeks. By 12 weeks, all the pituitary glands of M became prolactinomas, associated with increased serum PRL levels and the weight of pituitary gland in a time- and dose-dependent pattern. Conclusion Intraperitoneal DES injection can forming prolactinoma in Wistar rat. It is a convenient and stable pituitary tumor model. Gender difference and ovariectomy are not significantly different to forming prolactinoma.