苄基四氢帕马汀经蛋白激酶C对豚鼠心肌细胞延迟整流钾电流的作用及其临床应用(英文)

Influence of benzylenzyltetrahydropalmatine on the delayed rectifier potassium current by protein kinase C pathway in guinea pig ventricular myocytes

目的:研究苄基四氢帕马汀(BTHP)经蛋白激酶C对豚鼠心肌细胞延迟整流钾电流的作用极其在临床中的应用前景。方法:应用膜片钳在全细胞模式下记录延迟整流钾电流(Ik)。结果:PMA10.0μmol/L在细胞内给药可使Ik和Ik,tail增加,电流密度分别从(13.1±1.4)pA/pF和(5.1±0.7)pA/pF增至(19.5±0.7)pA/pF和(7.3±0.4)pA/pF。应用BTHP后上述增加效应被明显减少,BTHP的抑制作用在单独应用时为(38±6)%和(36±6)%,而在预先应用PMA后BTHP的抑制作用增加至(64±7)%和(64±6)%。结论:BTHP对Ik和Ik,tail的阻滞作用可能部分与抑制细胞内蛋白激酶C途径有关。

AIM:To investigate influence of BTHP on the delayed rectifier potassium current by protein kinase C pathway in Guinea pig ventricular myocytes and its prospect in clinic.METHODS:Ik was recorded using whole cell arrangement of the patch clamp procedure.RESULTS:Phorbol 12 myristate 13 acetate( PMA,10.0 μ mol/L) caused an enhance effect on Ik intercellularly, with increasing of Ik and Ik,tail from (13.1± 1.4) pA/pF and (5.1 ± 0.7 ) Pa/pF to (19.5± 0.7) pA/pF and (7.3± 0.4) pA/pF.Inhibition effect of BTHP on Ik was markedly augmented after pre application of PMA.Inhibition rates of Ik and Ik,tail were increased from (38± 6)% and (36± 6)% only with BTHP to (64± 7)% and (64± 6)% in the exposure to BTHP together with PMA.