一氧化氮合酶抑制剂基因在离心机训练大鼠各组织中的表达差异

Expression pattern of protein inhibitor of neuronal nitric oxide synthase gene in SD rats during centrifuge training

目的:观察神经元一氧化氮合酶抑制剂(PIN)基因在离心机训练大鼠各组织中的表达情况,探讨PIN基因表达在大鼠抗正向加速度(+Gz)负荷能力中的作用。方法:SD大鼠分别进行离心机训练和正向加速度(+Gz)耐力测试,将PIN基因片段用Dig-11-dUTP标记制成探针,用狭线杂交法分析大鼠各组织RNA中PIN基因的表达量。结果:PIN基因在离心机训练6,12d后心(F=5.444,P<0.05)、脑、肾(F=19.759,P<0.01)组织中的表达水平逐渐升高,而在肠、肺组织中的表达量呈下调趋势;与正常对照组相比,PIN基因在未训练测试组心(t=2.520,P<0.05)、脑(t=4.115,P<0.01)、肾(t=4.442,P<0.01)组织中的表达水平升高,而在肠、肺组织中呈下调趋势;训练12d后测试组与训练12d组相比,PIN基因在心、脑、肺和肠各组织中的表达无明显区别,在肾组织中的表达量明显降低。PIN基因在高、低耐力大鼠各组织中的表达水平无明显差别。结论:PIN基因表达升高可能是组织对抗+Gz负荷的生理代偿调节之一,以加强机体在细胞和分子水平对高+Gz应激环境的适应能力。

AIM:To demonstrate the expression of protein inhibitor of neuronal nitric oxide synthase(PIN)geue in specific tissues in centrifuge training rats and to investigate the effect of PIN gene expression in loading ability against positive acceleration(+ Gz). METHODS:Twelve SD rats with the weight of 200- 230 g were equally divided into three groups at random for centrifuge testing: normal control group, 6 hour trial group and 12 hour trial group.For + Gz tolerance testing other twenty five SD rats were randomly divided into two groups: non training group(n=15) and training group(n=10).SD rats were trained by centrifuge and/or exposed to + Gz tolerance testing.mRNA were extracted from rat heart,brain,kidney,lung and intestine,and gene PIN cDNA was labeled with Dig 11 dUTP.Its expression levels were measured by thin line hybridization. RESULTS:The expression levels of gene PIN in heart( P< 0.05) , brain and kidney( P< 0.01) of SD rats increased gradually following centrifuge training for 6d and 12d, but decreased in lung and intestine. Compared with normal control rats, gene PIN expression of heart( P< 0.05) , brain( P< 0.01) and kidney( P< 0.01) in non training group increased significantly, and decreased in lung and intestine. The PIN expression in heart, brain, lung and intestine had no significant difference between 6 hour and 12 hour training group, but its expression level in kidney in 12 hour trial group was significantly lower than that in training group. No significant differences of PIN expression levels were found between high and low + Gz tolerance rats.