尿脱落细胞吖啶橙染色诊断膀胱移行细胞癌的意义

The significance of acridine orange stain in the diagnosis of transition cell carcinoma of bladder

目的 探讨尿脱落细胞吖啶橙染色法(AO-F)阳性率与膀胱移行细胞癌之间的关系。方法 1993-01/2001-12因膀胱移行细胞癌住院手术患者60例,对肿瘤不同的分期、分级、大小、数目、部位来统计其AO-F的阳性率。结果 AO-F总阳性率为71.7％,浅表性膀胱癌和浸润性膀胱癌患者的AO-F阳性率分别为60.0％和88.0％;肿瘤分级Ⅱ级以下者AO-F阳性率41.7％,Ⅱ级或以上者AO-F91.7％;肿瘤直径≥2cm者AO-F阳性率为80.0％,<2cm者为63.3％.多发性和单发性膀胱癌AO-F阳性率分别为75.0％和70.0％;肿瘤累及膀胱三角区或膀胱颈部AO-F阳性率88.9％,未累及者68.6％。AO-F阳性者肿瘤复发率为48.8％,AO-F阴性患者52.9％。结论 AO-F阳性率与肿瘤的分期和分级有明显的相关性。肿瘤的大小、数目、部位及复发率和AO-F无明显相关性。

AIM To evaluate the significance of acridine orange stain (AO-F) in diagnosis of transitional cell carcinoma (TCC) of bladder. METHODS 60 patients with TCC of bladder were selected from 1993 to 2001.The positive rate of AO-F in different stages, grades, size, number, position of tumor were calculated. RESULTS The total positive rate of AO-F was 71.7%.The positive rate was 60.0% in Ta and T1 stage, 88.0% in invasive TCC, 41.7% in grade below Ⅱ 91.7% in grade Ⅱ or more than Ⅱ,80.0% in size of tumor more than 2 cm ,63.3% in size less than 2 cm,75.0% in multiple tumors,70.0% in single tumor,88.9% in tumor which involving trigone or neck of bladder,68.6% in tumor not in involving these regions respectively.The recurrence rate was 48.8% in patients with positive AO-F and 52.9% in patients with negative one. CONCLUSION A close association is observed between stage, grade of TCC and AO-F. There is no significant association between size, number, position of the tumor and A0-F,and between the recurrence of TCC and AO-F.