苏云金芽孢杆菌七个亚种酌DNA的G+C克分子百分数的测定

DETERMINATION OF MOLE PERCENT OF G+C IN DNA OF BACILLUS THURINGIENSIS SEVEN SUBSPECIES

苏云金芽孢杆菌在害虫的生物防治以及植物抗虫育种中的作用日益扩大。该类细菌资源的开发和利用越来越被重视,而正确的分类鉴定方法是进行该项研究的基础。目前苏云金芽孢杆菌的分类鉴定是根据形态特征、生理生化特性、鞭毛抗原(H)的血清型以及营养细胞的酯酶型等,将其分为31个亚种。随着分子生物学和遗传学的高速发展,

Mole percent of G + C in DNA of Bacillus thuringiensis seven subspcies were determinated by means of thermal denaturation method of nucleic acid. The result showed that:B, t. subsp, thuringiensis(H_1)' G+C% =32.5t B.t.subsp. finitimus (H_2) G+C%=31.0; B.t. subsp, alesti(H_(3a)) G+C%=30.0; B.t. subsp, Kurstaki(H_(3a3b)) G + C% = 30.03 B.t. subsp, kenyae (H_(4a4c)) G + C% = 29.5; B.t. subsp, galleriae(H_(5a5b)) G+C%=32.0; B.t. subsp, wenquanensis G+C%=31.5.