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胰岛素样生长因子结合蛋白-7基因的克隆和表达 被引量：1

Cloning and expression of IGFBP-7gene
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摘要 IGFBP-7是胰岛素样生长因子结合蛋白(IGFBP)家族中的一员,具有一些抑癌基因的特征。利用重组PCR技术获得了IGFBP-7基因的全长编码区序列,并将其克隆到pcDNA3.1/His-Myc真核表达载体中,得到重组质粒pcDNA3.1/His-Myc-IGFBP-7。将该重组质粒瞬时转染人胚胎肾293T细胞,Western-blot分析表明,IGFBP-7在293T细胞中获得了表达,为进一步研究IGFBP-7的功能奠定了基础。 IGFBP-7is a member of insulin-like growth factor-binding protein(IGFBP)family,which acts as a poten-tial tumor suppressor.In this study,full-length coding region of IGFBP-7gene was obtained by recombinant PCR,and cloned into the eukaryotic vector pcDNA3.1/His-Myc,resulting in recombinant plasmid pcDNA3.1/His-Myc-IGFBP-7.The recombinant plasmid was transiently transfected into human embryonic kidney293T cells.Western blot analysis showed that IGFBP-7was efficiently expressed in293T cells.The results lay foundations for further study on function of IGFBP-7.
作者 丁丽华 张京生 周围 钟红君 王宗华 黄翠芬 叶棋浓
机构地区 军事医学科学院生物工程研究所 福建农林大学 福建农林大学
出处 《生物技术通讯》 CAS 2003年第4期282-284,共3页 Letters in Biotechnology
基金 军事医学科学院启动基金 福建"百千万"人才工程项目
关键词 胰岛素样生长因子结合蛋白—7 基因 克隆 表达 insulin-like growth factor-binding protein-7 recombinant PCR cloning expression IGFBP-7
分类号 Q786 [生物学—分子生物学]
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参考文献7

  • 1Swisshelm K, Ryan K, Tsuchiya K, et al. Enhanced expression of an insulin growth factor-like binding protein(mac25)in senescent human mammary epithelial cells and induced expression with retinoic acid[J]. Proc Natl Acad Sci USA, 1995,9:4472.
  • 2Burger AM, Zhang X, Li H, et al. Down-regulation of T1A12/mac25, a novel insulin-like growth factor binding protein related gene,is associated with disease progression in breast carcinomas[J]. Oncogene, 1998,16:2459.
  • 3Murphy M, Pykett MJ, Harnish P, et al. Identification and characterization of genes differentially expressed in meningiomas[J]. Cell Growth Differ, 1993,4:715.
  • 4Landberg G, Ostlund H, Nielsen NH, et al. Down-regulation of the potential suppressor gene IGFBP-rP1 in human breast cancer is associated with inactivation of the retinoblastoma protein,cyclin E overexpression and increased proliferation in estrogen receptor negative tumors[J]. Oncogene, 2001,20:.3497.
  • 5Oh Y, Nagalla SR, Yamanaka Y, et al. Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7[J]. J Biol Chem, 1996,271:30322.
  • 6Sprenger CC, Damon SE, Hwa V, et al. Insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) is a otential tumor suppressor protein for prostate cancer[J]. Cancer Res,1999,59:2370.
  • 7Pereira RC, Canalis E. Parathyroid hormone increases mac25/insulin-like growth factor-binding protein-related protein-1 expression in cultured osteoblasts[J]. Endocrinology, 1999,140:.1998.

同被引文献26

  • 1Oh Y, Nagalla SR, Yamanaka Y, et al.Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein specifically binds IGF-Ⅰ and -Ⅱ[J].J Biol Chem, 1996,271(48): 30322-30325.
  • 2Pereira RC, Blanquaert F, Canalis E.Cortisol enhances the expression of mac25/insulin-like growth factor-binding protein-related protein-1 in cultured osteoblasts[J]. Endocrinology, 1999, 140(1):228-232.
  • 3Ahmed S, Yamamoto K, Sato Y, et al.Proteolytic processing of IGFBP-related protein-1 (TAF/angiomodulin/mac25) modulates its biological activity[J].Biochem Biophys Res Commun,2003,310(2):612-618.
  • 4Wixler V, Geerts D, Laplantine E, et al. The LIM-only protein DRAL/FHL2 binds to the cytoplasmic domain of several alpha and beta integrin chains and is recruited to adhesion complexes[J].J Biol Chem, 2000, 275(43): 33669-33678.
  • 5Wilson EM, Oh Y, Hwa V, et al.Interaction of IGF-binding protein-related protein 1 with a novel protein, neuroendocrine differentiation factor, results in neuroendocrine differentiation of prostate cancer cells[J].J Clin Endocrinol Metab, 2001 , 86(9):4504-4511.
  • 6Sanchez-Garcia I, Rabbitts TH. The LIM domain: a new structural motif found in zinc-finger-like proteins[J].Trends Genet,1994,10(9):315-320.
  • 7Curtiss J, Heilig JS.DeLIMiting development[J].Bioessays,1998,20(1):58-69.
  • 8Lee SM, Li HY, Ng EK, et al.Characterization of a brain-specific nuclear LIM domain protein (FHL1B) which is an alternatively spliced variant of FHL1[J].Gene, 1999, 237(1): 253-263.
  • 9Chan KK, Tsui SK, Lee SM, et al.Molecular cloning and characterization of FHL2, a novel LIM domain protein preferentially expressed in human heart[J].Gene,1998, 210(2): 345-350.
  • 10Lee SM, Tsui SK, Chan KK, et al.Chromosomal mapping of a skeletal muscle specific LIM-only protein FHL3 to the distal end of the short arm of human chromosome 1[J].Somat Cell Mol Genet,1998,24(3): 197-202.

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生物技术通讯
2003年 第4期

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