葡聚糖酶高产菌株的诱变筛选及其摇瓶发酵条件的初步研究

Study on screening a high-productive strain of dextranase and its basic conditions of flask fermentation

从土壤中分离出的一株产葡聚糖酶酶活31U/ml的野生菌株,经UV、60Co、LiCl诱变筛选后得到了产葡聚糖酶高产菌株SB126,经发酵条件优化试验后检测其酶活达到85U/ml,较野生菌株提高了近两倍。葡聚糖酶摇瓶较适发酵条件为:装量30ml(250ml)、转速180r/min、pH7.0、温度30℃、接种量8%、发酵周期5d。

A series of experiments were done to screen highly dextranase-productive strain by mutagenesis and study its basic fermentation conditions.Its original enzyme activity was31U/ml.Treated by UV,60 Co,LiCl mutagenesis,the highly productive strain SB126was finally obtained.The enzyme activity went up to85U/ml in the end.The basic fermentation conditions were flask content 30ml/250ml,rotation speed180r /min,pH7.0,temperature30℃,inoculating quantity8%,and5d.