ZT、ABA等内源物质与杉木树龄及其组培复壮的关系

Relationship between Endogenous ZT,ABA and Age of Chinese Fir as well as It's Rejevenation by Tissue Culture

对不同树龄的杉木嫩梢及其培养体中ABA、ZT和单宁含量的变化作了分析测定。结果表明:在不同季节ABA和单宁的含量均和树龄呈正相关。三种年龄杉木的嫩梢中ABA含量的年变化趋势基本相同,均以抽梢旺季较低,盛夏季节较高。根据ABA水平,组培材料的采集应在春梢生长旺季进行。 采自2、10年生母本的外植体材料,经初代培养后,ZT含量均有明显上升。但在2年生母本培养体中尤为明显。与此同时ABA含量却大幅度下降。经4次继代培养后两种材料中ZT含量均有所下降,且差异缩小。ABA含量却出现回升。它们的消长与嫩梢增殖能力有关。

The change on contents of ZT, ABA and tannin in shoot of material trees and their cultures in Chinese Fir (Cunninghamia lanceolate) were analyzed. Results show: the contents of ABA and tanin were both positively correlated to tree age at all seasons. Yearly fluctuation of ABA content showed almost identical in shoots from 2,10 and over 20-year-old material trees, which was the highest in peak periods of shoot growing and the lowest in midsummer. According to ABA level, The peak period in spring was the best time for collecting materials for culture in vitro. ZT content had a marked rise while ABA level dropped dramatically in explants both from 2-and-lo-year-old material trees after 3-month original culture. Such changes were more striking in explant from 2-year-old material. After four successive subculture lasted for one year, the ZT content declined, but their difference was reduced between cultures from trees at different ages. At the same time ABA content increased again. The changes of their contents affected the capacity of shoot multiplication during culture in vitro.