马尾松毛虫对氰戊菊酯的耐药性研究

Studies on the Insecticide-Tolerance of Dendrolimus punctatus to Fenvaleratc

应用生物测定技术和生理生化分析方法,对马尾松毛虫进行了耐药性研究,结果表明:①马尾松毛虫对氰戊菊酯的耐药力随龄期增加而增加,6龄幼虫耐药力为3龄幼虫的37倍;②常用拟除虫菊酯的铜山林区对马尾松毛虫尚未形成抗药性;③非专一性酯酶活力随龄期增加而增大,铜山种群酯酶活力较茅山种群为大,这与马尾松毛虫的耐药性水平相一致;④两地种群体壁抗穿透性无异质性。药剂穿透体壁动态研究表明:试虫受药后8h,仍有43.3％的氰戊菊酯未进入体内,体壁中残留的氰戊菊酯量为受药量的1.8％,表明体壁起了屏障作用。

The insecticide-tolerance of Dendrolimus punctatus Walker to fenvalerate was studied in Jiangshu Province through bioassay and the methods of physiology & biochemistry. The results showed that:(1) The insecticide tolerance to fenvalerate increased with the increase of instars and it was 37 times in the 6th instar larvae more than that of the 3rd instar; (2) The resistance to fenvalerate and deltamethrin had not formed yet in Tongshan colony; (3) The activity of carboxyesterase increased gradually with the increase of instars and was greater in Tongshan colony than that in Maoshan colony; (4) The results of dipping and injection showed less difference between the Tongshan and Maoshan colony. The dynamic study on pesticide-penetrating-cuticula of Tongshan colony showed that 43.3% fenvaleratc did not entered cuticula, and that 1.8% fenvalerate remained in integument 8 hours after dipping the larvae.