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杉木悬浮细胞系的建立和原生质体的分离 被引量:7

Establishment of Cell Suspension Culture and Isolation of Protoplast of Chinese fir
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摘要 山杉木(Cunninghamia lanccolata Hook.)成熟种子胚在含有2.0mg/l 2,4-D、0.2mg/l KT的MS培养基上诱导愈伤组织,通过筛选继代培养,得到了生长旺盛、质地松散的愈伤组织细胞系。继代初期适当降低蔗糖浓度有助于减少愈伤组织的褐化。将5个月龄的愈伤组织转移至含1.5~2.0 mg/l 2,4-D、0.1~0.2mg/l KT、200 mg/l CH的MS液体培养基振荡培养,建立了增殖快、分散性良好的杉木悬浮细胞系。从4个月龄的悬浮细胞酶解(2%纤维素酶、0.25%果胶酶和13%甘露醇),游离得到大量的杉木原生质体,产量为9.3×10~5个/g FWW,活力达到80%以上。 Callus was induced by embryos from mature seeds of Chinese fir (Cttnninghamia lanceolata Hook.), which had been cultured in MS medium containing 2.0mg/l 2,4-D and 0.2mg/l KT. After successive subculture and selection, cell colonies of the calli which were frible and in rapid growth were obtained. In the period of initial subculture, decrease of sucrose concentration was beneficial to diminishing callus browning. The cell suspension culture, which grew rapidly and consisted mainly of smaller cell aggregates, was established by transferring five-month-old calli to liquid MS medium supplemented with 1.5~2.0mg/l 2.4-D, 0.1~0.2mg/l KT and 200 mg/1 CH. A great amount of Chinese Fir protoplasts were released from the cell suspension cultures, with protoplast yield (9.3x105/g FW) and viability (above 80 %). Enzyme mixture was consisted of 2 % cellulase R-10, 0.25% pectinase and 13% mannitol.
出处 《林业科学研究》 CSCD 北大核心 1992年第6期628-632,共5页 Forest Research
基金 中国林科院及亚热带林业研究所科研基金
关键词 杉木 悬浮细胞系 原生质体的分离 Chinese Fir cell suspension culture protoplast isolation
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参考文献8

  • 1黄敏仁,许农,陈道明.林木原生质体培养的现状[J].植物生理学通讯,1990,26(2):75-78. 被引量:9
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二级参考文献5

  • 1S. M. Attree,F. Bekkaoui,D. I. Dunstan,L. C. Fowke. Regeneration of somatic embryos from protoplasts isolated from an embryogenic suspension culture of white spruce (Picea glauca)[J] 1987,Plant Cell Reports(6):480~483
  • 2Faouzi Bekkaoui,Praveen K. Saxena,Stephen M. Attree,Larry C. Fowke,David I. Dunstan. The isolation and culture of protoplasts from an embryogenic cell suspension culture of Picea glauca (moench) voss[J] 1987,Plant Cell Reports(6):476~479
  • 3Julie A. Russell,Brent H. McCown. Techniques for enhanced release of leaf protoplasts in Populus[J] 1986,Plant Cell Reports(4):284~287
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