Ki-67抗原在宫颈癌的表达和临床意义

EXPRESSION AND CLINICAL SIGNIFICANCE OF Ki-67 ANTIGEN IN INVASIVE CARCINOMA OF CERVIX

目的 :探讨Ki- 6 7抗原在宫颈癌的表达及临床意义。方法 :应用免疫组化技术检测 15例正常宫颈上皮(normalcervicalepithelium ,NCE) ,18例宫颈上皮内瘤样病变 (cervicalintraepithelialneoplasm ,CIN)和 75例宫颈浸润癌 (invasivecarcinomaofcervix ,ICC)Ki- 6 7的表达情况。结果 :Ki- 6 7阳性染色位于细胞核。Ki- 6 7在NCE、CIN和ICC的阳性表达率分别为 13.33%、72 .2 2 %和 96 .0 0 % ,从NCE到CIN再到ICC ,Ki- 6 7阳性表达率显著升高 (P <0 .0 1)。Ki- 6 7在ICC的表达与间质浸润有关 (P <0 .0 5 ) ;而与年龄、FIGO分期、组织学分级、组织学类型、盆腔淋巴结转移和脉管浸润无关 (P >0 .0 5 )。宫颈癌突破深层间质浸润者 ,其Ki- 6 7阳性表达率显著高于浸润深度在浅层间质以内者 (P <0 .0 5 )。结论 :Ki- 6 7抗原可能在宫颈癌发生发展和间质浸润过程中起重要作用。检测Ki- 6 7抗原的表达情况对于进一步了解宫颈癌生物学行为和判断预后有一定的应用价值。

Objective:To investigate the expression and clinical significance of Ki-67 antigen in cervical carcinoma .Methods: The expression of Ki-67 antigen in 15 cases of normal cervical epithelium (NCE), 18 cases of cervical intraepithelial neoplasm (CIN) and 75 cases of invasive carcinoma of cervix (ICC) was detected by immunohistochemistry SP method.Results: The staining reaction of Ki-67 was mainly confined to the cellular nucleus in tumor. It was showed that the rate of positive expression of Ki-67 in NCE, CIN and ICC was 13.33%, 72.22% and 96.00% respectively. The rate of positive expression of it increased remarkably in the order of NCE, CIN and ICC ( P <0.01) .The expression of Ki-67 in ICC was correlated with stroma involvement ( P <0.05), and the rate of the staining reaction of Ki-67 in cases with deep stroma involvement was remarkably higher than that in the cases without it ( P <0.05). There was no relationship between the expression of Ki-67 and age, the international federation of gynecology and obstetrics staging (FIGO staging), histological grading, histological types, pelvic lymph node metastasis or intravascular involvement ( P >0.05).Conclusion:Ki-67 antigen may play an important role during the canceration, cancer progression and stroma involvement of cancer cells in cervical carcinoma. Detection of it may be valuable to further understand the biological behavior of cervical carcinoma and estimate its prognosis.