参麦对休克复苏时肠黏膜内一氧化氮、丙二醛及钙含量的影响

PROTECTIVE EFFECT OF SHENMAI INJECTION ON INTESTINAL MUCOSA DURING REPERFUSION:AN EXPERIMENT STUDY

目的 :观察参麦注射液 (简称参麦 )对休克复苏期间肠黏膜内一氧化氮 (NO)、丙二醛 (MDA)及钙 (Ca2 + )含量的影响 ,探讨其肠黏膜保护作用机制。方法 :采用Lamson兔休克肠缺血再灌注模型 ,2 1只家兔随机分为对照组 (A组 )、单纯复苏组 (B组 )和参麦治疗组 (C组 )。C组于复苏同时首剂静注参麦 2 .1mg/kg ,随后持续泵入 7.5ml/kg·h ,直至观察结束。分别于放血前 (N)、休克 1h(S1)、再灌注 1h(R1)和 3h(R3 )观察参麦对乙状结肠黏膜内pH(pHi)及R3 时回肠黏膜NO、MDA及Ca2 + 含量的影响。结果 :C肠黏膜NO、MDA及Ca2 + 显著低于B组 (均为P <0 .0 5 ) ,其中Ca2 + 与A组无显著性差异 ,而NO及MDA含量高于A组 ,R1和R3 时肠 pHi明显高于B组 ;B组肠黏膜NO、MDA及Ca2 + 含量明显高于A组和C组 (P <0 .0 5 ) ,R1～R3 期间肠 pHi持续低水平。结论 :参麦减轻肠黏膜再灌注损伤与其改善黏膜灌注及氧合、抑制NO的产生或释放、降低氧自由基水平和防止钙超载有关。

Objective:To investigate the effect of Shenmai on nitric oxide(NO),malondialdehyde(MDA) and Ca 2+ contents of intestinal mucosa during reperfusoin in shocked rabbits and study the mechanism of protective effect of Shenmai.Methods:Twenty-one rabbits were divided randomly into 3 groups (n=7):control group (A),shock-reperfusion group(B),Shenmai group(C).According to Lamson method,the model of intestine ischemia/reperfusion in rabbits was established.Intestinal intramucosal pH(pHi) of sigmoid colon,the content of NO,MDA and Ca 2+ in intestine mucosa were measured.Results:NO,MDA and Ca 2+ contents of intesinal mucosa in group C were significantly lower than those in group B respectively.NO,MDA contents of intestinal mucosa in group C were higher than those in group A,while Ca 2+ content of intesinal mucosa remained unchanged in group C as compared with that in group A.PHi of sigmoid colon was increased markedly at 1h and 3h of reperfusion in group C.NO,MDA and Ca 2+ contents of intestinal mucosa in group B were significantly higher than those in group A and C.Conclusions:Shenmai injection can protect intestine mucosa from reperfusion damage by improving perfusion and oxygentation of intestinal mucosa,inhibiting the production or release of NO and reducing oxygen free radicals and calcium overload.