雌激素对去势大鼠颌骨和股骨结构影响的实验研究

Effects of estrogen on the structure of mandible and femur in ovariectomized rats

目的 :观察雌激素对实验性骨质疏松大鼠颌骨和股骨结构影响的相互关系。方法 :切除SD雌性大鼠双侧卵巢建立骨质疏松动物模型 ,给予腹腔注射雌二醇 ,治疗 6周后处死大鼠 ,取颌骨和股骨标本 ,对组织切片进行骨组织形态计量学分析。结果 :去势后的大鼠颌骨、股骨骨小梁呈疏松化改变 ,股骨骨量减少明显多于颌骨 ;雌二醇治疗后可明显抑制颌骨、股骨骨质的吸收 ,颌骨骨量变化更为明显。结论 :雌激素治疗对于骨质疏松的颌骨、股骨骨小梁结构的影响是有差异的 ,治疗效果颌骨优于股骨 ,可能与扁骨。

Objective: To investigate the effects of estrogen (E 2) on the structure of madible and femur in ovariectomized rats. Methods: 18 femal SD rats were evenly divided into 3 groups:sham-ovariectomized rats (sham-OVX),ovariectonmized rats without E 2 treatment (osteoporosis,OP) and ovariectomized rats with E 2 treatment (E 2).In the group of E 2,E 2 at the dose of 10 μg/kg was injected into abdominal cavity of the rats every the other 2 days.All rats were sacrificed after 6-week treatment.The madible and femur samples were analyzed by bone histomorphometry with QWin550CW system. Trabecular area (Tb.Ar,%), trabecular width (Tb.Wi,μm) and trabecular seperation (Tb.Sp,μm) were calculated.Result: Tb.Ar(%),Tb.Wi (μm) and Tb.Sp(μm) in the madibles in sham-OVX rats were 29.43±3.12,10.97±1.85 and 27.59±4.21;in OP 19.44±2.24,6.45±1.73 and 42.03±5.46;in E 2 treated 26.81±2.93,9.54±1.22 and 30.98±3.65,respectively.Those in the femus in sham-OVX rats were 24.19±5.53,56.72±10.36 and 132.41±47.69;in OP 12.54±4.32,40.71±8.65 and 208.67±65.39;in E 2 treated 20.08±4.86,51.38±9.07 and 159.42±56.34,respectively.Conclusion: The bone volume decreases more in femur than in madible after ovariectomy in rats.Treament of estrogen can inhibit bone absorbtion in mandible and femur.