丹参对缺血心肌细胞L-型钙电流的影响

Effect of Danshen on L-type Calcium Channel Current in Isolated Myocardiocytes After Ischemia

目的 :观察丹参对缺血心肌细胞L 型钙电流 (ICa L)的影响。方法 :应用膜片钳全细胞记录技术。结果 :5 0 ,10 0 ,2 0 0mg/L丹参分别将缺血心肌细胞ICa L 的峰值从 (6 5 9.7± 14 8.5 ) pA降至 (5 31.9± 113.2 ) pA、(492 .4± 4 9.3) pA、(341.8± 72 .6 )pA(n =8,P <0 .0 5或P <0 .0 1)。结论 :丹参能有效地减少缺血心室肌细胞的ICa L 。

Objective:To investigate the effect of Danshen on L-type calcium channel current(I Ca-L ) in isolated myocardiocytes after ischemia. Method:Whole cell patch clamp technique was employed. Result:In isolated myocardiocytes after ischemia, Danshen 50,100,200 mg/L reduced the I Ca-L from (659.7±148.5)pAto (531.9±113.2),(492.4±49.3), (341.8±72.6) pA (n=8,P<0.05 or P<0.01)respectively.Conclusion:Danshen can significantly reduce the I Ca-L in isolated myocardiocytes after ischemia, which may contribute to its effect on anti-myocardial ischemia and ischemia-induced arrhythmia.