乙型肝炎患者肝组织中HBcAg特异性T细胞克隆的建立及莫特异性分析

Characterization of T Cell Clones with Specificity for Hepatitis B Virus Nucleoprotein Derived from Liver Tissue of Patients with Chronic Hepatitis B

用γHBcAg通过有限稀释法从慢性乙肝(CHB)患者肝组织浸润淋巴细胞中分离出12个HBcAg特异性T细胞克隆,均为CD_3^+CD_4^+CD_3^-TCRα/β^+HLA-DR^+表型。对其中3个克隆的抗原特性及主要组织相容复合体(MHC)的限制性进行了分析,克隆对HBcAg呈剂量依赖关系,而对HBsAg、破伤风类毒素(TT)及纯蛋白衍生物(PPD)无反应性。克隆细胞对HBcAg的特异性增殖反应需要抗原提呈细胞(APC)表面HLA-DR 1分子的存在;同时抗MHCⅡ类抗原单克隆抗体能明显抑制克隆增殖反应。

Hepatitis B virus nucleoprotein (HBcAg)-specific T cell clones were established from lymphocytes infiltrating the liver by culturing with autologous feeder cells and rHBcAg. All these clones had the CD3^(+) CD4^(+) TCRa/β^(+) HLA-DR^(+) phenotype. of 12 clones 3 were analyzed in detail. Antigenic specificity was confirmed by dose-dependent, HLA class Ⅱ (DR_1)-restricted proliferation in response to γHBcAg, but not to HBsAg. HBcAg-specific helper T cells may play a main role in inducing antigen-specific functional responses by autologous B and T lymphocytes at the site of viral antigen synthesis and hepatocellular injury in HBV infection.