肝癌和癌周双份肝组织的HBV DNA整合状况

Molecular Status of Hepatitis B Virus DNA in Paired Tumorous and Nontumorous Tissue from 30 Cases of Hepatocellular Carcinoma

本文对30例肝癌患者的肝组织进行研究,从其中24例HBsAg(+)病人检出HBVDNA整合者21例,其中癌组织有整合者18例,癌周组织有整合者15例,双份组织均有整合者13例。6例HBsAg(-)者的1例癌周组织也检出HBVDNA整合。杂交带分析发现,不同病例和同一例肝内不同组织整合带数目和电泳位置不一致。23例癌周肝组织有明显的碎屑坏死,这可能与整合、癌变有关。

The molecular status of hepatitis B virus (HBV) DNA in 30 paired tumorous and nontumorous liver tissue from 24 HBsAg-seropositive and 6 HBsAg-negative patients with HCC were studied by a modified Southern blot. Twenty-one integrants discovered including 20 HBsAg- positive and 1 HBsAg--negative, among which 18 harour integrated HBV DNA in tumorous tissue, 16 in nontumorous and 13 in both. The bands and locations of integrated HBV DNA were different between different tissues as well as were among different integrants suggesting the randonness and rearrangement of the integration. Since the probe contains a sequence from 1437bp to 2809bp in HBV genome, the result may reflect the status of S gene and its downstream promotor. By pathological examination, 23 patients had been found to have piecemeal necrosis in their nontumorous tissue, which may be a coincidence with HBV integration.