净化烹饪油污微生物菌种的选育

Microbial screening and culture on cooking oil waste

利用正交试验方法优化了净化烹饪油污菌种选育的环境和营养条件 ,即 :油 :2 0mL、NaNO3( 2g L) :4mL、KH2 PO4( 0 .5g L) :3mL、微量元素溶液 (CuSO4·5H2 O 0 .0 3g L ,ZnSO4·7H2 O 0 .1g L ,MnCl2 ·4H2 O 0 .0 5g L) :1mL和温度 3 7℃ ;而且得出氮源是影响菌体增长的最重要因素 ,温度是影响微生物降解的重要因素 ,而微量元素和磷源对菌种培育结果影响较小。在此优化条件下选育出了合适降解烹饪油污的c种子菌液 ,其每天可降解油 2 2mg L以上 ,并鉴定出主要的微生物是嗜水性气单孢菌 (Aeromonashydrophila 4AK4)、黄丝藻属 (Tribonema)和多甲藻属 (Peridinium)等菌体 ;分析了选育过程活性微生物、目标污染物和微生物的代谢产物三者之间的关系 。

By orthogonal experiment method, the environmental and nutritional conditions of the microbial screening and culture for the degradation of cooking oil waste were optimized : oil waste 20 mL, NaNO 3(2 g/L) 4 mL, KH 2PO 4(0.5 g/L) 3 mL, trace element(CuSO 4·5H 2O 0.03 g/L, ZnSO 4·7H 2O 0.1 g/L, MnCl-2·4H 2O 0.05 g/L) 1 mL and temperature at 37℃. Source of nitrogen was confirmed to be the most important factor affecting the microbial growth, and temperature was the another important factor affecting the microbial degradation; however, trace element and source of phosphor had less influence to the microbial screening and culture. Under the optimal conditions, inoculum c was screened and cultured and could effectively degrade cooking oil waste to 22 mg/L·d. Inoculum c was identified as Aeromonas hydrophila 4AK4,Tribonema and Peridinium. By analyzing the concentration relationship among oil, vigor microbes and extracellular polymers in the microbial screening and culture for the degradation of cooking oil waste, it showed microbes can use oil waste as source of the C and breed themselves and discharge metabolic product.