韭不同倍性及其非整倍体的ITS序列变异研究

Studies on Variation of ITS Sequences with Different Ploidy and Its Aneuploid in Allium tuberosum

对不同倍性及其非整倍体韭Alliumtuberosum和野韭Alliumramosum核糖体DNA的内部转录区 (ITS)进行了PCR扩增和克隆 ,并测定 10个ITS1和ITS2的序列 ,用ITS1+ITS2的序列探讨Alliumtuberosum和Alliumramosum的系统发育关系。研究表明 ,Alliumtuberosum与Alli umramosum种间的平均Kimura遗传距离仅为 0 0 14 6 ,二者是一对近缘种。虽然韭经历了长期的人工选择和自然选择 ,但与野韭的分化仍然较小。韭不同倍性及其非整倍体之间 ,其ITS序列亦无明显差异。

The ITS regions of 2 closely related species Allium tuberosum Rottler. ex Spreng.and Alllium ramosum L. in Allium Sect. Rhiziridium G. Don were amplified, cloned and sequenced. The genetic relationships among 10 materials of diploid,triploid,tetraploid and aneuploid in Allium tuberosum and Alllium ramosum were reconstructed based on ITS1+ITS2 sequences. The results demonstrated that the average Kimura pairwise distance between Allium tuberosum and Allium ramosum was 0.0146.They are a pair of closely related species. Although it was gone through evolution by artificial selection and natural selection, Allium tuberosum kept little differentiation from Allium ramosum .Sequences of ITS region show that there are no evident differentiation among the various diploid,triploid,tetraploid and aneuploid of Allium tuberosum .