稗草致病菌——尖角突脐孢菌菌株RAPD指纹图谱的分析

Analysis of RAPD Fingerprinting in Exserohilum monoceras Strains

以我国主要稻区的稗草植株上分离的 1 7株尖角突脐孢菌菌株为试验材料 ,采用改良的SDS法提取其基因组DNA ,并运用优化的RAPD分析体系对其进行了分子标记遗传差异研究。从 2 5个随机引物中筛选出 2 0个扩增效果好的引物 ,对全部试验材料进行了RAPD扩增 ,共得到 2 39条有效带 ,其中多态性带 2 2 9条 (占 95 .8% )。依据扩增结果建立了 1 7株尖角突脐孢菌基因型的DNA指纹图谱并对其进行了有效区分。根据RAPD分析结果计算了菌株间的遗传距离 ,分析了它们的遗传差异并进行了聚类分析 ,结果表明 ,RAPD分子标记技术是能够用于杂草致病菌资源的鉴定的 ,并可以进一步应用于特定性状的基因标记研究。

Exserohilium monoseras is a potential agent for barnyardgrass (Echinochlao crus-galli) control. This study was to evaluate the genetic diversity of this fungus with 17 strains isolated from different rice growing regions of China by the random amplified polymorphic DNA (RAPD). Among 25 arbitrary primers, 20 primers could get enough amplified bands for all the strains. A total 239 products were amplified. Polymorphic bands were 95.8% of the total products. The 17 strains could be identified based on RAPD fingerprinting established in this study. Genetic distance calculation and cluster analysis resulted in great genetic variation among the strains. The genetic similarity was related with the morphology and the pathogenicity of the strains. The results showed that RAPD maker could be applied in the utilization and the identification of weed pathogen resources.