氧化亚铁硫杆菌分离复壮及固定化的研究

Study on the Rejuvenating by Isolation and the Immobilization of Thiobacillus Ferrooxidans

用稀释涂布平板法从已退化的氧化亚铁硫杆菌 (Thiobacillusferrooxidans)菌液中分离出氧化活性较高、生命力强的氧化亚铁硫杆菌T1。以H 2软性填料作为氧化亚铁硫杆菌的固定化载体 ,构建了固定床生物反应器。考察了固定床生物反应器氧化Fe2 + 的情况 :Fe2 + 最大氧化速率达 7 67g (L·h)。并对固定床生物反应器运行过程中在载体表面形成的沉淀物进行了研究 ,通过X衍射证明此沉淀物为黄钾铁矾 [KFe3(SO4 ) 2 (OH) 6 ]。

A strain of Thiobacillus ferrooxidation T1 with stronger oxidation ability and more exube- rant vitality was isolated from the liquid with regressive Thiobacillus ferrooxidans by method of dilution butteron on plate. A fixed-bed bioreactor was constructed by immobilized cells of Thiobacillus ferrooxidans with H-2 carriers. In the state of bioreactor operation,a maximum Fe 2+ oxidation rate of 7.67g[Fe 2+ ]·h -1 ·L -1 was attained.We studied the precipitates formed on the surface of the carriers during the operation of the bioreactor.By X-ray diffraction analyses we proved the precipitates were jarosite.