基因多态性与血清p53蛋白过表达的关系

Relationship between gene polymorphism and serum p53 protein overexpression

目的 探讨细胞色素P4 5 0 1A1(cytochromeP4 5 0 1A1,CYP1A1)和谷胱甘肽转硫酶M1(GlutathioneS -trans ferasesM1,GSTM1)基因多态性与血清p5 3蛋白过表达之间的关系。方法 以 12 7名血清p5 3蛋白过表达阳性者为病例组 ,以 12 7名血清p5 3蛋白阴性者为对照组 ,分别采用多重差异PCR(multi-differentialPolymerasechainreaction ,MD -PCR)检测外周血淋巴细胞GSTM1的缺失和采用等位特异PCR(allele -specificPoly -merasechainreaction ,AS -PCR)检测外周血淋巴细胞CYP1A1基因的等位变异 ,采用 χ2 检验来比较GSTM1和CYP1A1的多态性的差异。结果 单独分析CYP1A1和GSTM1基因多态性在 2组中的分布没有显著性差异 (P >0 0 5 ) ,但GSTM1基因缺失型即GSTM1(- )和CYP1A1基因纯合突变型即CYP1A1Val/Val基因型联合增加了血清p5 3蛋白过表达的危险性 (P <0 0 5 ) ,OR及 95 %CI为 3 86 (0 95～ 15 5 9)。结论 CYP1A1和GSTM1的等位变异联合增加了血清p5 3蛋白过表达的危险性。

Objective To explore relationship between gene polymorphism of GSTM1,CYP1A1 and serum p53 protein overexpression. Methods Case group was 127 people whose serum p53 protein was overexpression,and control group was 127 people without serum p53 protein overexpression.Polymorphism of Glutathione Stransferases M1(GSTM1) gene in peripheral leukocytes was detected by multi-differential polymerase chain reaction(MD-PCR).The polymorphism of cytochrome P4501A1(CYP1A1) gene in peripheral leukocytes was detected by allele-specific polymerase chain reaction(AS-PCR).Relationship between polymorphism of GSTM1 and CYP1A1 and serum p53 protein overexpression was evaluated by pearson Chi-Square. Results When relationship between overexpression of serum p53 protein and polymorphism of CYP1A1 and GSTM1 were analysed respectively,no significant difference were found.But combined effect of GSTM1 null and CYP1A1 Val/Val increased risk in overexpression of serum p53 protein(P<0.05,OR=3.86,95%CI:0.95-15.59). Conclusion Combined effect of variation of GSTM1 and CYP1A1 increased risk in overexpression of serum p53 protein.