Kato-Katz与醛醚法定性定量诊断血吸虫病的对比分析

Comparison of Kato-Katz and formol-ether concentration method in diagnosis of schistosomiasis japonica

目的评价Kato-Katz与醛醚法检测日本血吸虫病定性与定量的效果。方法以Kato-Katz与醛醚法联合检出的38例日本血吸虫卵阳性患者的结果为金标准,将Kato-Katz与醛醚法的检测结果分别与金标准进行比较。结果 Kato-Katz与醛醚法的虫卵阳性检出率分别为81.58%和89.47%,经四格表的确切概率法检验,差异无统计学意义(P=0.795);阳性患者的平均EPG,Kato-Katz法为醛醚法的3.6倍;但Kato-Katz法的材料费与标本制作费比醛醚法低2.33倍。结论日本血吸虫卵的检出率Kato-Katz与醛醚法相近,但血吸虫病感染度,Kato-Katz法的EPG值远高于醛醚法,在进行血吸虫病的定量研究时,应采用醛醚法为宜。

Objective To evaluate the qualitative and quantitative effect of Kato- Katz and formolether concentration methods in diagnosis of Schistosomiasis japonica. Methods Kato-Katz and formol-ether concentration methods were respectively used to determine the findings by comparing with the positive results of Schistosoma japonicum eggs detected by the combined use of the two approaches in 38 cases as gold standard.Results Positive egg rate by Kato-Katz and formol-ether concentration technique was respectively 89.47%and 81.58%, and the difference was not significant after verification of the exact probability by fourfold and contingency table(P = 0.795). By mean eggs per gram(EPG) of stool from the positively infected patients, the count by Kato-Katz method was 3.6 times of that by formol-ether concentration technique, and the cost of materials and specimen preparation was 2.33 times lower in Kato-Katz method than formol-ether concentration. Conclusion Kata-Katz method has similar detection rate to formol-ether concentration for Schistosoma japonicum eggs, yet the former can result in higher EPG value in determination of the infection density than the latter. Given quantitative study of schistosomiasis, formol-ether concentration is appropriately recommended.