3株白纹伊蚊生物学特性及对寨卡病毒易感性的比较

Biological characteristics and susceptibility of three Aedes albopictus strains to Zika virus

目的探究3个地理株白纹伊蚊的生物学特性及其对寨卡病毒(ZIKV)的易感性差异。方法建立广州株(GZ株)、深圳株(SZ株)和恩平株(EP株)白纹伊蚊,观察记录3株蚊株的生长发育周期;检测3个蚊株Wolbachia携带率和其组织分布;经口感染ZIKV后,检测不同地理株白纹伊蚊对ZIKV的易感性。结果 3个地理株白纹伊蚊生长发育周期无显著差异且均携带Wolbachia,但SZ株和GZ株白纹伊蚊组织内Wolbachia含量高于EP株,差异有统计学意义(P<0.05)。经口感染ZIKV后,3个地理株的白纹伊蚊均可有效传播ZIKV,GZ株白纹伊蚊对ZIKV的感染率最高为91.7%,传播率为66.7%;EP株白纹伊蚊对ZIKV的传播能力最弱,传播率为54.5%。结论不同地理株白纹伊蚊对ZIKV的易感性不同,可能与其组织内Wolbachia含量有关。

Objective To determine the biological characteristics of three geographically Aedes albopictus strains and their susceptibility to Zika virus(ZIKV).Methods Three strains of Aedes albopictus,Guangzhou(GZ),Shenzhen(SZ)and Enping(EP)were established in the laboratory.The infection rate and somatic tissue distribution of Wolbachia in three mosquito strains were detected.The oral susceptibility of local Aedes albopictus to ZIKV was measured.Results There was no significant difference in the growth cycle among three geographic Aedes albopictus strains.All of them were naturally super infected with Wolbachia,w AlbA and w AlbB,but Wolbachia density in the somatic tissue of Aedes albopictus SZ strain and GZ strain were significantly higher than that of EP strain(P<0.05).After oral infection to Asian ZIKV,three geographic strains of Aedes albopictus could effectively transmit ZIKV.GZ strain was the most susceptible to ZIKV with infection rate91.7%and transmission rate 66.7%.However,the EP strain showed weak transmission ability(54.5%)to ZIKV.Conclusion The susceptibility of three Aedes albopictus strains to ZIKV is different and may be correlated to the Wolbachia density in mosquito tissues.