摘要
目的探讨miR-449a、miR-26a、CXC趋化因子配体5(CXCL5)在溃疡性结肠炎组织中的表达水平及其预后研究。方法选取2017年5月至2018年4月本院进行治疗的94例溃疡性结肠炎患者,以及同期行单发息肉切除的72例肠粘膜组织正常者进行研究,所有受试者均行结肠镜检查采集结肠粘膜组织,实时定量逆转录聚合酶链式反应(qRT-PCR)检测组织中miR-449a、miR-26a表达水平,免疫组化法检测组织中CXCL5阳性表达情况,分析miR-449a、miR-26a和CXCL5与溃疡性结肠炎患者病理参数的关系;采用COX回归分析、KM曲线分析miR-449a、miR-26a和CXCL5与溃疡性结肠炎患者预后的关系。结果与正常组比较,溃疡性结肠炎组中miR-449a、miR-26a表达水平下降,CXCL5阳性表达率升高,差异有统计学意义(P<0.05)。miR-449a、miR-26a和CXCL5表达与患者疾病活动程度、内镜分级、组织学分级相关,差异有统计学意义(P<0.05)。COX单因素分析显示,疾病活动程度、内镜分级、组织学分级、miR-449a、miR-26a、CXCL-5与患者预后有关,差异有统计学意义(P<0.05);多因素显示,疾病活动程度、内镜分级、miR-449a、miR-26a和CXCL5为影响患者预后的独立危险因素,差异有统计学意义(P<0.05)。miR-449a、miR-26a低表达组和CXCL5高表达组黏膜愈合良好率分别为49.09%、49.06%和48.28%,均低于miR-449a、miR-26a高表达组与CXCL5低表达组的71.79%、76.73%和75.00%,差异有统计学意义(P<0.05)。结论miR-449a、miR-26a在溃疡性结肠炎患者中低表达,CXCL5呈高表达,三者与患者黏膜组织预后情况有关,且为黏膜愈合不良的独立危险因素。
Objective To investigate the expression levels and their clinical significances of miR-449 a,miR-26 a and CXC chemokine ligand 5(CXCL5)in ulcerative colitis.Methods Ninety-four patients with ulcerative colitis and 72 patients with normal intestinal mucosal tissues who underwent single polypectomy were included;colonoscopy was performed to collect colonic mucosal tissues for all the subjects.The expression levels of miR-449 a and miR-26 a in the tissues were detected by using real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR),the positive expression of CXCL5 was detected by immunohistochemistry,the relationships between miR-449 a,miR-26 a,CXCL5 and pathological parameters of patients with ulcerative colitis were analyzed,and the relationships between the levels of miR-449 a,miR-26 a,CXCL5 and the prognosis of patients with ulcerative colitis were analyzed by COX regression analysis and KM curve analysis.Results Compared with the normal group,the expression levels of miR-449 a and miR-26 a were decreased and the positive expression rate of CXCL5 was increased in the ulcerative colitis group(P<0.05).Correlation analysis showed that signifcant positive correlations existed between the expressions of miR-449 a,miR-26 a,CXCL5 and disease activity,endoscopic grading,histological grading(P<0.05).Univariate analysis of COX showed that correlations existed between disease activity,endoscopy grading,histological grading and prognosis.Ultivariate analysis showed that disease activity,endoscopy grading,miR-449 a,miR-26 a and CXCL5 were independent factors influencing the prognosis of patients.The mucosal healing rates in miR-449 a and miR-26 a low expression groups and CXCL5 high expression group were lower than those of miR-449 a and miR-26 a high expression groups and CXCL5 low expression group(P<0.05).Conclusion The expressions of miR-449 a and miR-26 a were decreased and CXCL5 was increased in patients with ulcerative colitis,which were all independent risk factors for poor mucosal healing and may be related to the prognosis of mucosal tissues.
作者
徐彦玲
时霞
张立辉
XU Yan-ling;SHI Xia;ZHANG Li-hui(Rizhao Hospital of Traditional Chinese Medicine,Rizhao,Shandong 276800,China)
出处
《热带医学杂志》
CAS
2019年第7期880-885,942,共7页
Journal of Tropical Medicine