Th22细胞及相关细胞因子在慢性乙型肝炎肝硬化患者中的表达及意义

Significance of Th22 cells and the repression of related cytokines in patients with chronic hepatitis B cirrhosis

目的探讨Th22细胞及相关细胞因子在慢性乙肝肝硬化(LC)患者中的表达及意义。方法选取本院2014年8至2017年9月确诊为慢性乙型肝炎患者(CHB)25例、LC患者40例及正常对照者(HC)25例。分别检测清晨空腹外周血Th22细胞比例,白细胞介素-22(IL-22)的浓度及相关细胞因子白细胞介素-6(IL-6),肿瘤坏死因子(TNF-α),芳香烃受体(AHR),维甲酸相关孤儿核受体C(RORC)等在各组患者中的表达。结果 LC组和CHB组Th22细胞占比明显高于HC组,且LC组明显高于CHB组,差异有统计学意义(P<0.05)。LC组和CHB组丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和DNA载量均高于HC组,且CHB组高于LC组,差异均有统计学意义(P<0.05)。CHB组和HC组血清IL-22浓度显著低于LC组,各组间比较差异有统计学意义(P<0.05)。LC组IL-22、IL-6、TNF-α、AHR和RORC基因的相对表达量分别为(2.21±1.58)、(6.23±4.25)、(1.62±0.25)、(4.20±1.27)和(4.58±2.12),均高于CHB组和HC组,差异有统计学意义(P<0.05);CHB组IL-22、IL-6、TNF-α、AHR和RORC基因的相对表达量均高于HC组,差异有统计学意义(P<0.05)。LC组IL-22细胞数(65.32±34.19)个/hpf显著高于HC组(4.12±2.15)个/hpf和CHB组(33.02±6.01)个/hpf,差异有统计学意义(P<0.05)。结论 Th22细胞参与了LC的发生和发展,可能是预防和治疗肝硬化的重要靶点。

Objective To investigate the significance of Th22 cells and the expression of related cytokines in patients with chronic hepatitis B liver cirrhosis(LC).Methods 25 cases of chronic hepatitis B(CHB),40 cases of LC and 25 cases of healthy control(HC)confirmed in our hospital from 2014 to 2017 were selected.The precentage of Th22 cells,the levels of plasma interleukin-22(IL-22)and related cytokines IL-6,tumor necrosis factor-α(TNF-α),aryl hydrocarbon receptor(AHR)and retinoic acid-related orphan nuclear receptor C(RORC)in all groups of patients were detected.Results The percentages of Th22 cells in LC group and CHB group were significantly higher than that in HC group;the percentages of Th22 cells in LC group was significantly higher than that in CHB group(P<0.05).The alanine aminotransferase(ALT)level,aspartate transaminase(AST)level and DNA load in LC group and CHB group were higher than those in HC group(P<0.05),and those in CHB group were higher than those in LC group(P<0.05).The plasma IL-22 concentrations in CHB group and HC group were significantly lower than that in LC group(P<0.05).The relative expression levels of IL-22,IL-6,TNF-α,AHR and RORC gene in LC group were(2.21±1.58),(6.23±4.25),(1.62±0.25),(4.20±1.27)and(4.58±2.12)respectively,significantly higher than those in CHB group and HC group(P<0.05).The relative expression levels of IL-22,IL-6,TNF-α,AHR and RORC genes in CHB group were significantly higher than those in HC group(P<0.05).The number of IL-22 cells in LC group(65.32±34.19)/hpf was significantly higher than those in HC group(4.12±2.15)/hpf and CHB group(33.02±6.01)/hpf(P<0.05).Conclusion Th22 cells were likely involved in the occurrence and development of LC and might be an important target for the prevention and treatment of liver cirrhosis.