强直性脊柱炎患者外周血单核细胞miR-138表达及与Th1/Th2失衡的关系

Expression of miR-138 in peripheral blood mononuclear cells of patients with ankylosing spondylitis and its relationship with Th1/Th2 imbalance

目的检测强直性脊柱炎(AS)患者外周血单核细胞(PBMCs)miR-138表达水平及Th1/Th2比例,并分析二者之间的关系。方法选取2016年2月-2018年2月本院确诊的AS患者97例,根据病情活动指数(BASDAI评分)分为活动组与稳定组,选取本院同期健康体检者100例作为对照组,利用流式细胞术检测Th1、Th2细胞比例,采用ELISA法检测研究者血清样本中干扰素-γ(IFN-γ)、肿瘤坏死因子(TNF)、白细胞介素-4(IL-4)、IL-12水平,利用实时定量PCR(q RT-PCR)法检测PBMCs中miR-138表达情况,利用全自动生化分析仪检测血清C反应蛋白(CRP)、利用魏氏法检测红细胞沉降率(ESR)、利用流式细胞仪检测人白细胞抗原B27(HLA-B27)。结果 AS患者血清CRP水平、ESR速率显著高于对照组,差异有统计学意义(P<0.05);与对照组比较,活动组AS患者miR-138表达水平降低,差异有统计学意义(P<0.05),Th1细胞亚群比例、Th1/Th2比例、血清IFN-γ、IL-4、IL-12水平升高,差异有统计学意义(P<0.05),稳定组miR-138表达水平降低(P<0.05),Th1细胞亚群比例、Th1/Th2比例、IL-4、IL-12水平升高,差异有统计学意义(P<0.05);与稳定组比较,活动组AS患者miR-138表达水平降低,IFN-γ水平升高,差异有统计学意义(P<0.05)。AS患者PBMCs中miR-138与Th1/Th2比值负相关(P<0.05)。结论 AS患者PBMCs中miR-138表达水平降低,Th1/Th2比值升高,二者呈负相关。

Objective To detect the expression level of miR-138 and the ratio of Th1/Th2 in peripheral blood mononuclear cells(PBMCs)of patients with ankylosing spondylitis(AS),and to analyze the relationship between them.Methods 97 patients with AS diagnosed in our hospital from February 2016 to February 2018 were selected,according to the disease activity index(BASDAI score),the patients were divided into active group and stable group;100 health examinees in our hospital during the same period were selected as control group.The proportion of Th1 and Th2 cells was detected by flow cytometry,the levels of interferon-γ(IFN-γ),tumor necrosis factor(TNF),interleukin-4(IL-4)and IL-12 in serum samples were detected by ELISA;real-time quantitative PCR(q RT-PCR)was used to detect the expression level of miR-138 in PBMCs;serum C-reactive protein(CRP)was detected by automatic biochemical analyzer;erythrocyte sedimentation rate(ESR)was measured by Wechsler method,and human leukocyte antigen B27(HLA-B27)was detected by flow cytometry.Results The serum CRP level and ESR rate of AS patients were significantly higher than those of control group(P<0.05);compared with the control group,the expression level of miR-138 in AS patients in the active group decreased(P<0.05);the percentage of Th1 cell subsets,the ratio of Th1/Th2,levels of serum IFN-γ,IL-4 and IL-12 increased(P<0.05);the expression level of miR-138 in the stable group decreased(P<0.05);the percentage of Th1 cell subsets,Th1/Th2 ratio,IL-4 and IL-12 levels increased(P<0.05);compared with the stable group,the expression level of miR-138 in AS patients in active group decreased(P<0.05);the level of IFN-γincreased(P<0.05).MiR-138 was negative correlated with Th1/Th2 ratio in PBMCs of AS patients(P<0.05).Conclusion The expression level of miR-138 in PBMCs of AS patients was decreased,and Th1/Th2 ratio was increased;there was a negative correlation between them.