DHEA对辐射所致小鼠DNA损伤防护作用的研究

Study of the DHEA Protective Effect on DNA Damage Induced by Radiation in Mice

目的探讨DHEA(17 aα-D-高炔雌二醇-3-乙酯)对辐射导致小鼠DNA损伤的防护作用。方法 IRM-2近交系小鼠照射前3 d、2 d、1 d连续3次给予DHEA,用单细胞凝胶电泳技术,检测2 Gyγ射线辐照后小鼠淋巴细胞DNA双链断裂。结果 DHEA低、中、高各剂量组、炔雌醇组(EE2)、尼尔雌醇组(523)的彗星尾部(TDNA%)、彗星尾长(TL)、尾矩(TM)和Olive尾矩(OTM)等指标的数值明显低于对照组,与对照组比较差异有统计学意义(P<0.01),DHEA中剂量组的TDNA、TL、TM和OTM等指标的数值明显低于EE2组和523组,与EE2组和523组比较差异有统计学意义(P<0.01)。结论 DHEA能明显减轻淋巴细胞DNA双链断裂损伤,对辐射导致小鼠DNA损伤具有明显的保护作用。

Objective To investigate the protective effects of the DHEA( 17 a α-D-homo ethynycl dstradid-3-accetale) on DNA damage in mice lead by radiation. Methods IRM-2 inbred mice injected with DHEA consecutively three times 3d、2d、1d before irradiation. Neutral single cell gel electrophoresis was used,and DNA double strand breaks of the mouse lymphocytes were detected after 2 Gy gamma ray irradiation. Results The index illustrated by comet tail DNA content( TDNA%),comet tail length( TL),tail moment( TM) and Olive tail moment( OTM) in DHEA groups with low,middle and high doses,ethinyl estradiol( EE2) group and Neal estradiol( 523) group were significantly lower than those of the control group. There was statistically significant difference compared with the control group( P < 0. 01). The index demonstrated by TDNA,TL,TM and OTM in the DHEA middle dose group was significantly lower than that of EE2 and the 523 groups. And there were significant differences compared with the 523 and EE2 groups( P < 0. 01). Conclusion DHEA can significantly reduce the lymphocyte DNA double strand breaks and has obvious protective effect on mice DNA damage caused by radiation.