解郁颗粒对抑郁模型大鼠血清中皮质醇及海马环磷酸腺苷含量的影响

Effect of Jieyu Granules on the Contents of Cortisol and Hippocampal Cyclic Adenosine Monophosphate in Serum of Model Rats with Depression

目的:观察院内制剂解郁颗粒对抑郁模型大鼠血清中皮质醇(Cortisol,CORT)及海马环磷酸腺苷(Cyclic adenosinemonophosphate,cAMP)含量的影响并探讨其作用机制。方法:将72只雄性SD大鼠分为空白组、模型组、盐酸氟西汀组、解郁颗粒大、中、小剂量组。采用慢性轻度不可预见性的应激抑郁大鼠模型,观察大鼠体质量及行为学变化,给药21天后,测定血清中CORT及海马c AMP含量。结果:实验前各组大鼠体质量均无明显差异(P> 0.05)。在第22天,各组大鼠体质量均有增加,与空白组比较,模型组大鼠体质量较小(P <0.05);水平运动、垂直运动得分及海马cAMP水平明显减少,CORT水平明显升高,差异均有统计学意义(P <0.01)。与模型组比较,盐酸氟西汀组、解郁颗粒大、中剂量组体质量显著升高(P <0.01,P <0.05);盐酸氟西汀组、解郁颗粒各剂量组大鼠的水平运动和垂直运动得分均较高(P <0.05);CORT水平明显下降,海马cAMP水平明显升高,差异均有统计学意义(P <0.01,P <0.05)。结论:解郁颗粒对抑郁模型大鼠有抗抑郁的作用,其作用机制可能是通过纠正下丘脑-垂体-肾上腺轴(HPA轴)亢进,从而上调AC-cAMP-PKA信号通路实现的。

Objective:To observe the effect of Jieyu granules,the hospital preparation,on the contents of cortisol(CORT)and hippocampal cyclic adenosine monophosphate(cAMP) in serum of model rats with depression and to explore its mechanism. Methods:Divided 72 male SD rats into six groups,namely the blank group,the model group,the fluoxetine hydrochloride group, the Jieyu granules groups of high-dose, medium-dose and low-dose. Adopted model rats with depression of chronic unpredictable mild stress,and observed the changes in the body mass and behavior of the rats. After 21-day drug administration, measured the contents of CORT and hippocampal cAMP in serum. Results: Before the experiment,there was no significant difference in the body mass of each group(P > 0.05). On the 22nd day,the body mass of rats in each group increased,compared with that in the blank group,the body mass of the model group was smaller(P <0.05);the scores of horizontal and vertical movements and the level of hippocampal cAMP were obviously decreased,while the level of CORT was obviously increased,differences being significant(P < 0.01). Compared with the model group,the body mass of the fluoxetine hydrochloride group and the Jieyu granules groups of high-dose and medium-dose were obviously increased(P < 0.01,P < 0.05);the scores of horizontal and vertical movements of the fluoxetine hydrochloride group and the Jieyu granules groups of each dose were higher(P < 0.05);the levels of CORT were obviously decreased,while the levels of hippocampal cAMP were obviously increased, differences being significant(P < 0.01, P < 0.05).Conclusion:Jieyu granules have good antidepressant effect on model rats with depression. Its mechanism is probably to correct the hyperfunction of the hypothalamus-pituitary-adrenal axis(HPA axis),thereby up-regulating the AC-cAMP-PKA signal pathway.