黄芪保心汤对心肌梗死后心衰大鼠心室重构及相关炎性因子影响的研究

Study on the Effect of Huangqi Baoxin Tang on Ventricular Remodeling and Related Inflammatory Factors in Rats with Heart Failure After Myocardial Infarction

目的:探讨黄芪保心汤对心肌梗死后心衰大鼠心室重构及改善心功能的作用。方法:将120只SD大鼠随机分为假手术组(20只)和造模组(100只),假手术组仅穿线,不结扎前降支(LAD),模型组结扎LAD,饲养4周后,假手术组剩余14只,模型组通过彩超验证造模成功65只,随机分为:卡托普利组给予6.75 mg/(kg·d)的卡托普利,黄芪保心汤低、中、高剂量组分别给予5.67 g/(kg·d)、11.34 g/(kg·d)、22.68 g/(kg·d)的黄芪保心汤,模型组给予等量蒸馏水,每组13只。喂药4周后,观察大鼠一般情况,用酶联免疫吸附实验(ELISA)法来检测各组大鼠血清N末端脑钠肽前体(NT-ProBNP)、白细胞介素-6 (IL-6)、肿瘤坏死因子-α(TNF-α)、心肌基质金属蛋白酶-2 (MMP-2)、基质金属蛋白酶-9 (MMP-9)的变化;通过HE染色观察心肌组织形态结构改变。结果:与假手术组比较,模型组大鼠血清NT-proBNP、IL-6、TNF-α、MMP-2、MMP-9数值均升高(P <0.01);心肌细胞排列紊乱,大量纤维组织增生。与模型组比较,黄芪保心汤各组均可以不同程度地降低血清NT-pro BNP、IL-6、TNF-α、MMP-2、MMP-9含量(P <0.05,P <0.01);心肌细胞排列整齐、纤维组织增生减轻(P <0.05,P <0.01)。黄芪保心汤低、中、高剂量组呈现量效正向关系。结论:黄芪保心汤对心梗后心衰大鼠炎症因子IL-6、TNF-α和心肌基质金属蛋白酶MMP-2、MMP-9有调控作用,可改善心脏形态结构和心肌重构,提高心功能。

Objective:To investigate the effect of Huangqi Baoxin tang on ventricular remodeling and cardiac function improvement in rats with heart failure after myocardial infarction. Methods:Divided 120 SD rats randomly into the sham operation group(20 rats) and the modeling group(100 rats). The sham operation group was only given threading and did not ligature the left anterior descending(LAD),and the model group had LAD ligated. After 4 weeks of feeding,14 rats remained in the shame group,and 65 models were successfully completed in the model group by color Doppler ultrasound. These 65 rats were randomly divided into the captopril group given 6.75 mg/(kg·d) of captopril and the groups of low-dose,medium-dose and high-dose of Huangqi Baoxin tang given 5.67 g/(kg·d),11.34 g/(kg·d),22.68 g/(kg·d) of Huangqi Baoxin tang,respectively;and the model group was given equal amount of distilled water,13 rats in each group. After 4 weeks of drug administration,observed the general conditions of rats;detected the changes of serum N-terminal pro-brain natriuretic peptide(NT-ProBNP), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α), myocardial matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) by applying enzyme-linked immunosorbent assay(ELISA);and observed the changes of myocardial tissue morphology by HE staining. Results:Compared with the sham operation group,the values of serum NT-proBNP,IL-6,TNF-α,MMP-2,and MMP-9 in the model group were increased(P < 0.01);the myocardial cells were in a disorder and a large number of fibrous tissues proliferated. Compared with the model group,the amount of serum NT-pro BNP,IL-6,TNF-α,MMP-2 and MMP-9 in groups of Huangqi Baoxin tang with different dose was decreased at different degree(P < 0.05, P < 0.01);the myocardial cells were arranged in line and the fibrous tissue proliferation was alleviated(P < 0.05,P < 0.01). There was a positive dose-effect relationship showing in the groups of low-dose,medium-dose and high-dose of Huangqi Baoxin tang. Conclusion:The application of Huangqi Baoxin tang can regulate inflammatory factors of IL-6 and TNF-α,myocardial matrix metalloproteinases of MMP-2 and MMP-9 in rats with heart failure after myocardial infarction. It can improve cardiac morphological structure and myocardial remodeling, and strengthen cardiac function.