氟达拉宾预防移植物抗宿主病的实验研究

Study on prophylactic effect of fludarabine in graft-versus-host disease

目的 研究氟达拉宾在大鼠异基因骨髓移植模型中对移植物抗宿主病 (GVHD)的预防作用。方法 Wistar大鼠为供鼠 ,SD大鼠为受鼠 ,受鼠分为 4组 (A、B、C、D) ,分别予以不同的预处理。A、B组各鼠自移植前 1～ 4d腹腔注射氟达拉宾 (1mg/kg) ,移植前 4h分别接受剂量为 2、6Gy的全身照射 ;C、D组仅在移植当天分别接受 2、6Gy的全身照射。各组受鼠均在移植当天经尾静脉输注供鼠骨髓细胞 3.8× 10 7个。观察各组大鼠GVHD反应。结果 移植前经氟达拉宾预处理的大鼠较单用全身照射预处理的大鼠生存期明显延长 ,且临床、病理GVHD评分也较低。结论 氟达拉宾可有效预防GVHD 。

Objective To study the prophylactic effect of fludarabine in graft versus host disease (GVHD) after bone marrow transplantation in rat models. Methods SD rats were transplanted with Wistar rats allogeneic bone marrow transplantation model. SD rats were divided into 4 groups. In group A and B, fludarabine was given as prophylaxis against GVHD. Group A and B were under condition with fludarabine for 4 days at a dose of 1 mg/kg per day 1 prior to transplantation, and received TBI 2 Gy and 6 Gy 4 hours prior to BMT; Group C and D were exposed to doses of 2 Gy and 6 Gy TBI at the same time. All these recipient rats were injected with donor bone marrow cells at a dose of 3.8×10 7 bone marrow cells per mouse by tail vein. Afterwards we observed GVHD of the four groups. Results Survival periods of rats received fludarabine were longer than that of rats only received TBI ( P <0.05), and the severity of graft versus host disease was also milder. Conclusion Fludarabine can effectively prevent GVHD and alleviate complication associated with allogeneic bone marrow transplantation.