黄芪—丹参配伍对腺嘌呤诱导慢性肾功能衰竭大鼠心血管钙化的影响

Effects of Compatibility of Radix Astragali and Radix Salviae Miltiorrhizae on Cardiovascular Calcification in Adenine-induced Renal Failure Rats

【目的】观察黄芪—丹参配伍应用对慢性肾功能衰竭(肾衰)大鼠心血管钙化的影响。【方法】将25只大鼠随机分为空白组,模型组,培哚普利组,黄芪—丹参低、高剂量组,每组5只。除空白组外,其余各组均采用腺嘌呤喂饲法建立肾衰大鼠模型。各给药组在造模同时开始给药干预。培哚普利组给予培哚普利叔丁胺片(剂量为0.42 mg·kg-1·d-1)灌胃,黄芪—丹参低、高剂量组给予黄芪—丹参水煎提取液(剂量分别为1.0、4.0 g·kg-1·d-1)灌胃。共给药4周。给药结束后分别测定大鼠体质量,左心室指数,血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)及血肌酐(SCr)、血尿酸(SUA)水平,主动脉组织钙离子(Ca2+)、碱性磷酸酶(ALP)含量及血清成纤维细胞生长因子23(FGF-23)水平。【结果】与空白组比较,模型组大鼠体质量降低,左心室指数升高,SCr水平升高,主动脉组织Ca2+含量显著降低、ALP含量升高,血清FGF-23水平升高(P <0.05),但SUA水平无明显变化(P> 0.05);与模型组比较,黄芪—丹参低、高剂量组大鼠SCr水平、主动脉ALP含量、血清FGF-23水平降低(P <0.05),但体质量、左心室指数、主动脉组织Ca2+含量均无明显差异(P> 0.05)。【结论】黄芪—丹参配伍通过降低ALP、FGF-23水平可能控制肾衰大鼠心血管钙化。

Objective To observe the effect of compatibility of Radix Astragali and Radix Salviae Miltiorrhizae(Huangqi-Danshen)on cardiovascular calcification of the adenine-induced chronic renal failure rats. Methods Twenty-five rats were randomly divided into 5 groups,namely blank group,model group,Perindopril group,low-and high-dose Huangqi-Danshen groups. A rat model of renal failure was established by adenine feeding methodin the rats except for the blank group. The 3 medicaiton groups were given the medicine at the same time for modeling.Perindopril group was given intragastric administration of Perindopril(at the dosage of 0.42 mg·kg-1·d-1),thelow-and high-dose Huangqi-Danshen groups were given Huangqi-Danshen Decoction(at the dosage of 1.0,4.0 g·kg-1·d-1),the treatment covering 4 weeks. After medication,we observed the body mass,left ventricleindex, the levels of serum alanine aminotransferase(ALT) and aspartate amino transferase(AST), serumcreatinine(SCr),serum uric acid(SUA),the contents of calcium ions(Ca2+)and alkaline phosphatase(ALP)inaorta tissues,and serum level of fibroblast growth factor 23(FGF-23). Results Compared with the blank group,the body mass was decreased,the left ventricle index was increased,SCr level was enhanced,the Ca2 +contentwas reduced and ALP content was increased in aortic tissues,and the serum FGF-23 level was increased in themodel group(P < 0.05),but SUA level had no obvious changes(P > 0.05). Compared with the model group,SCrlevel,ALP content,and FGF-23 level in aortic tissues was decreased(P < 0.05),while the body mass,the leftventricle index,Ca2+content in aortic tissues had no obvious changes(P > 0.05). Conclusion The compatibility of Huangqi-Danshen has probable effects on reliving the cardiovascular calcification of the renal failure rats throughdecreasing ALP and FGF-23 levels.