胡芦巴中皂苷成分的研究.新皂苷A及其次生苷的分离纯化和化学结构测定

Studies on saponin from seeds of Trigonella foenum-graecum (Ⅰ) Isolation and structural elucidation for a new saponin A and its secondary glucosides

目的 对胡芦巴 Trigonella foenum-graecum种子所含的皂苷成分作深入研究。方法 采用大孔吸附树脂法提取纯化总皂苷 ,并应用硅胶 H柱层析、干柱层析等方法分离出单一皂苷成分 ,通过 1 3 CNMR,FAB-MS,DEPT等光谱及部分水解获得的次生苷的方法进行了结构测定。结果 从总皂苷中分出一个新皂苷 A,为测定其结构将其部分水解获得次生苷 , 。经测定皂苷 A的化学结构为薯蓣皂苷元 -3 -O-α-L -鼠李吡喃糖 (1→ 4) -β-D-葡萄吡喃糖 (1→ 4) -β-D-葡萄吡喃糖苷 ;次生苷 结构为 :薯蓣皂苷元 -3 -O-β-D-葡萄吡喃糖苷 ;次生苷 结构为薯蓣皂苷元 -3-O-β-D-葡萄吡喃糖 (1→ 4) -β-D-葡萄吡喃糖苷。结论 皂苷

Object To investigate the saponin from the seeds of Trigonella foenum graecum Linn (STFG) Methods The total saponin from STFG was extracted and purified by using the absorptive resin, the single saponin was isolated by using the column chromatography as well as dry column chromatography of silica gel H The chemical structure was elucidated by 13 CNMR , FAB MS, DEPT spectroscopic evidence and the results of fraction hydrolysis of acquiring their secondary glucosides Results A new saponin A from the total saponin has been obtained, the fraction hydrolysis carried out and the secondary glucoside Ⅰ and Ⅱ identified by determining the structure of saponin A The chemical structure of saponin A is: diosgenin 3 O α L rhamnopyranosyl(1→4) β D glucopyranosyl(1→4) β D glucopyranoside The secondary glucoside Ⅰ is: diosgenin 3 O β D glucopyranoside; Ⅱ is: diosgenin 3 O β D glucopyranosyl(1→4) β D glucopyranoside Conclusion Glucoside A is a new saponin with three molecules of sugar