诱变剂亚硝基胍对海洋微藻卡德藻细胞形态及生长的影响

EFFECTS OF MUTAGEN MNNG ON CELL MORPHOLOGICAL CHANGE AND GROWTH OF MARINE MICRO ALGA Tetraselmis sp.

利用化学诱变剂亚硝基胍(MNNG)处理海洋单细胞绿藻卡德藻(Tetraselmis sp.),观测其对细胞形态和生长的影响。结果表明:MNNG导致卡德藻细胞出现鞭毛脱落、细胞膨胀、内部结构松散、胞质分布不均匀、色素体异常、产生休眠孢子以及致畸、致死等现象;MNNG对卡德藻在群体水平上的生长有明显的抑制作用,细胞死亡率与诱变剂剂量正相关,MNNG对卡德藻处理120 min的半致死浓度LC_(50)为20μg/L。

The effects of mutagen MNNG on marine microalgae Tetraselmis sp. were studied in this experiment. Microalgal cells were treated for 120 minutes under different concentration of MNNG (80,40,20,10,5,0 μg/L). Results showed that Te-traselmis sp. were sensitive to MNNG, morphological abnormality or even dead cells were observed, and growth was inhibited as well. LC50 of MNNG to Tetraselmis sp. at 120 min was 20 μg/L, and this dose can be act as an optimal one in the operation of inducing mutagenesis. It suggests that MNNG is an effective mutagenic agent in microalgal breeding and genetics study.