镉对中华绒螯蟹(Eriocheir sinensis)抗氧化酶活性的影响

EFFECT OF CADMIUM ON ANTIOXIDANT ENZYME ACTIVITIES OF THE JUVENILE Eniocheir sinensis

将中华绒螯蟹(河蟹Eriocheir sinensis)浸泡在2.0mg/L氯化镉(CdCl_2)溶液中,分别于第0、6、12、24、48、72、96小时提取河蟹的肝胰腺,测定抗氧化酶:超氧化物歧化酶(Superoxidedismutase,SOD)、过氧化氢酶(Catalase,CAT)、谷胱甘肽过氧化物酶(Glutathione Peroxidase,GPx)的活性,探讨了镉(Cd^(2+))对河蟹肝胰腺抗氧化酶活性的影响。结果显示,在低浓度Cd^(2+)环境下,河蟹肝胰腺的抗氧化酶活性随时间发生规律性变化。暴露于Cd^(2+)后,河蟹肝胰脏SOD活性降低,随时间的延长,在暴露72h后,SOD活性恢复并超过未染毒时22.3％。表明Cd^(2+)中毒导致细胞内氧自由基大量积累从而诱导酶活性升高;CAT活性先下降后增高,随后减少,最后以低于对照的水平趋于平衡;肝胰腺中GPx酶活性的变化规律与SOD相似,在解除氧自由基毒性方面有一定的协调性。实验表明河蟹抗氧化系统酶对Cd^(2+)很敏感。而酶活性在短时间内的变化规律可以为镉对河蟹早期的毒性研究提供参考。

Immersing the juvenile crabs (Eniocheir sinensis) in 2.0 mg/ L CdCl2 water, antioxidant enzyme activities of hep-atopancrea were assayed at 6, 12, 24.48, 72 and 96 h.The results showed that the enzyme activities were all decreased at first. The activity of SOD was increased 22.3% compared with control group at 72 h ; The activity of CAT was increased at 48 h, then it was decreased again, at last it got the horizontality. The change of the activity of GPx was just the same as the SOD. It is concluded that the antioxidant enzyme activities of hepatopancrea were highly sensitive to Cd2 +. So they have indicating values for Cd water pollution in early stages.