摘要
目的 :应用基因表达谱芯片探讨本芴醇衍生物LY980 5 0 3对肿瘤多药耐药逆转机制。方法 :提取多药耐药细胞株MCF/DOX总RNA ,与含有 32 0人基因的cDNA芯片杂交。结果 :有 9条基因表达下调 ,1条基因表达上调。结论 :LY980 5 0 3在体外能通过调节多种基因的表达而逆转MCF/DOX的耐药性。
AIM: To investigate the effect of LY980503(a benflumetol derivative)on multidrug resistance of tumor cell line using DNA microarray. METHODS: Total RNA was extracted from multidrug resistant MCF/DOX cell line. cDNA microarray containing 320 cDNAs was used to detect the gene expression profile. RESULTS: 9 down-regulated genes and 1 up-regulated gene were identified after multidrug resistant MCF/DOX cells were treated with LY980503. CONCLUSION: LY980503 can effectively reverse the resistance of MCF/DOX to DOX in vitro by adjusting the expression of multi-genes.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第8期1045-1048,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目 (No .3980 0 181)
关键词
寡核苷酸序列分析
乳腺肿瘤
基因表达
抗药性
多药
Oligonucleotide array sequence analysis
Breast neoplasms
Gene expression
Drug resistance, multiple
