急性坏死性胰腺炎肠粘膜损害与NK-IR的过度表达相关

Overexpression of neurokinin-1 receptor is associated with mucosal damage in acute necrotizing pancreatitis

目的 :研究神经激肽 - 1受体 (NK - 1R)和神经激肽 - 2受体 (NK - 2R)在急性坏死性胰腺炎 (ANP)大鼠末端回肠组织中的表达 ,探讨该受体的表达与ANP肠粘膜损害的关系。方法 :健康成年Sprague -Dawley大鼠随机分为假手术对照组 (5 0只 )和ANP组 (80只 )。假手术对照组开腹后只翻动胰腺 ,ANP组大鼠胰胆管恒速逆行注射 5 %牛磺胆酸钠 ,制成ANP大鼠模型。应用逆转录聚合酶链反应 (RT -PCR)检测末端回肠组织NK - 1R和NK - 2R的mRNA水平 ,应用Westernblot检测NK - 1R的蛋白表达水平。结果 :与假手术对照组相比 ,ANP末端回肠组织中NK - 1R和NK - 2R的mRNA水平过度表达。NK - 1R的表达水平分别与肠粘膜的病理学评分 (r =0 77,P <0 0 1)和肠粘膜通透性 (r=0 6 8,P <0 0 1)呈明显正相关。结论 :ANP时 ,肠组织中NK - 1R和NK - 2R的表达水平明显上调 ,P物质的过度作用加剧ANP时肠粘膜的病理损害 ,损害肠粘膜屏障的功能。

AIM: To study the expression and the role of neurokinin-1 receptor(NK-1R) and neurokinin-2 receptor (NK-2R) in distal ileum during acute necrotizing pancreatitis(ANP).METHODS: 130 adult Sprague-Dawley rats were divided into 2 groups: the rats in ANP group( n=80 ) were induced by the retrograde intraductal infusion of 5% sodium taurocholate. The rats in sham operation control group ( n=50 ) received laparotomy only. Reverse transcription polymerase chain reaction (RT-PCR) was used to determine the mRNA expression of NK-1R and NK-2R, Western blot was used to investigate expression level of NK-1R.RESULTS: NK-1R and NK-2R mRNA levels were enhanced in the distal ileum of ANP rats compared with controls. Western blot revealed stronger NK-1R immunoreactivity exited in intestinal mucosa in ANP rats. The overexpression of NK-1R was associated with mucosal pathological score( r=0.77,P<0.01 ) and intestinal permeability( r=0.68, P<0.01 ) in ANP. CONCLUSIONS: In ANP, overexpression of NK-1R and NK-2R contributes to disrupted neuropeptides loop balance, deteriorated intestinal injury, and be involved in intestinal hyperpermeability.