双歧杆菌对实验性大肠癌NF-κB和IκBα的影响

Influence of bifidobacterium on NF-κB and I κBα in experimental large bowel carcinoma

目的 :阐明青春型双歧杆菌的体内抑瘤机理。方法 :以大肠癌裸鼠移植瘤为动物模型 ,用激光共聚焦显微镜和免疫组化检测了大肠癌组织NF -κB及其抑制性蛋白IκBα的活性。结果 :双歧杆菌注射组大肠癌移植瘤NF -κB的阳性细胞密度明显低于肿瘤对照组 (P <0 0 1) ;而IκBα的表达则相反 ,双歧杆菌注射组大肠癌IκBα的平均荧光强度显著高于肿瘤对照组 (P <0 0 1)。结论 :青春型双歧杆菌体内能抑制大肠癌IκBα的降解 ,进而阻止NF -κB的活化。

AIM: To explore the antitumor mechanisms of bifidobacteria adolescence in vivo. METHODS: The activity of NF-κB and its inhibiting protein I κBα of large bowel carcinoma tissues was detected by using laser scanning confocal microscope and immunohistochemistry. RESULTS: The positive cell density of NF-κB of large bowel carcinoma transplantation tumors in bifidobacterium injection group was markedly lower than that in tumor control group( P< 0.01).The expression of I κBα was contary in the two group. The average fluorescent strength of I κBα of large bowel carcinoma in bifidobacterium injection group was significantly higher than that in tumor control group( P< 0.01). CONCLUSION: Bifidobacteria adolescence could inhibit the degrade of I κBα and the activition of NF-κB in large bowel carcinoma in vivo.