锌型复合无机抗菌材料的制备及性能研究

Preparation and Properties of Zinc Composite Inorganic Antibacterial Material

运用溶胶-凝胶法制备出二氧化硅载体,采用液相浸渍法制备得到锌型复合无机抗菌材料。结合响应曲面方法,考察了Zn2+浓度、p H和Tb3+浓度对材料抗菌率的影响,选取了较优制备条件:Zn2+浓度为0.3 mol·L^(-1),pH值为9,Tb3+浓度为0.01 mol·L^(-1)。以大肠杆菌为菌种,通过稀释涂布平板法,对材料进行抗菌性能检测,抗菌结果表明,掺杂Tb3+后材料抗菌率从76%提高到97%,较优样的最小杀菌浓度为5.5 g/L。通过XRD、FTIR、SEM、EDS和BET方法对材料的结构进行表征,结果表明:载体二氧化硅为无定形态,材料中主要的抗菌成分是纤锌矿结构的Zn O,且呈絮状和花瓣状生长,锌元素质量分数为16.96%,掺杂铽元素后材料的比表面积明显增大,增加了材料与细菌的接触面积,从而抗菌性能显著提高。

Silica was prepared by sol-gel method.Zinc-type inorganic composite antibacterial material was prepared by liquid dipping method.And the effect of three factors:zinc ion concentration,p H and terbium ion concentration on bactericidal rate were investigated through the response surface,the better preparation conditions were obtained,the concentration of zinc ion 0.3 mol·L^-1,p H was 9,the concentration of terbium ion 0.01 mol·L^-1.Escherichia coli was the experimental object.The antibacterial activity of the material was tested by LB-agar plates.The results show that the antibacterial rate of the material after adding rare earth terbium increased from 76%to 97%,and the best materials had a minimum bactericidal concentration of 5.5 g/L The materials were characterized by XRD,FTIR,SEM,EDS and BET.The results show that the silica was amorphous state.The main antibacterial component in the material was wurtzite-structured Zn O,and the shape of Zn O was flocculent and petal.Zinc weight fraction was 16.96%.The surface area of the material added with rare earth terbium was significantly increased,area of contact with bacteria increased too,meanwhile,the antibacterial performance increased.