摘要
运用溶胶-凝胶法制备出二氧化硅载体,采用液相浸渍法制备得到锌型复合无机抗菌材料。结合响应曲面方法,考察了Zn2+浓度、p H和Tb3+浓度对材料抗菌率的影响,选取了较优制备条件:Zn2+浓度为0.3 mol·L^(-1),pH值为9,Tb3+浓度为0.01 mol·L^(-1)。以大肠杆菌为菌种,通过稀释涂布平板法,对材料进行抗菌性能检测,抗菌结果表明,掺杂Tb3+后材料抗菌率从76%提高到97%,较优样的最小杀菌浓度为5.5 g/L。通过XRD、FTIR、SEM、EDS和BET方法对材料的结构进行表征,结果表明:载体二氧化硅为无定形态,材料中主要的抗菌成分是纤锌矿结构的Zn O,且呈絮状和花瓣状生长,锌元素质量分数为16.96%,掺杂铽元素后材料的比表面积明显增大,增加了材料与细菌的接触面积,从而抗菌性能显著提高。
Silica was prepared by sol-gel method.Zinc-type inorganic composite antibacterial material was prepared by liquid dipping method.And the effect of three factors:zinc ion concentration,p H and terbium ion concentration on bactericidal rate were investigated through the response surface,the better preparation conditions were obtained,the concentration of zinc ion 0.3 mol·L^-1,p H was 9,the concentration of terbium ion 0.01 mol·L^-1.Escherichia coli was the experimental object.The antibacterial activity of the material was tested by LB-agar plates.The results show that the antibacterial rate of the material after adding rare earth terbium increased from 76%to 97%,and the best materials had a minimum bactericidal concentration of 5.5 g/L The materials were characterized by XRD,FTIR,SEM,EDS and BET.The results show that the silica was amorphous state.The main antibacterial component in the material was wurtzite-structured Zn O,and the shape of Zn O was flocculent and petal.Zinc weight fraction was 16.96%.The surface area of the material added with rare earth terbium was significantly increased,area of contact with bacteria increased too,meanwhile,the antibacterial performance increased.
作者
毛华明
张彬
唐晓宁
蒋先发
叶孟婕
杨苏娥
MAO Hua-ming;ZHANG Bin;TANG Xiao-ning;JIANG Xian-fa;YE Meng-jie;YANG Su-e(Faculty of Science,Kunming University of Science and Technology,Kunming 650500,China;Faculty of Chemical Engineering,Kunming University of Science and Technology,Kunming 650500,Chin)
出处
《人工晶体学报》
EI
CAS
北大核心
2019年第3期505-513,共9页
Journal of Synthetic Crystals
基金
国家重点研发计划重点专项(2017YFC0210303)
昆明理工大学分析测试基金(2017T20030013)
关键词
二氧化硅
锌
铽
溶胶-凝胶法
抗菌材料
silica
Zinc
Terbium
sol-gel method
antibacterial material