曲利本红与氨基糖苷类抗生素相互作用的共振瑞利散射光谱及其分析应用

Resonance Rayleigh Scattering Spectra of Interaction of Aminoglycoside Antibiotics with Trypan Red and Their Analytical Applications

在弱酸性条件下 ,酸性双偶氮染料曲利本红 (TR)或硫酸卡那霉素 (KANA)、硫酸新霉素 (NEO)、硫酸庆大霉素 (GEN)和硫酸妥布霉素 (TOB)等氨基糖苷类抗生素的各自共振瑞利散射 (RRS)十分微弱 ,但两者相互作用形成离子缔合物时能使RRS急剧提高并产生新的RRS光谱 ,在 40 0～ 5 3 5nm之间有一个强的散射带 ,最大散射峰位于 40 0nm处 ,在 0 0 13～ 6 0 μg·mL-1范围内RRS强度与抗生素浓度成正比 ,可用于氨基糖苷类抗生素的测定 .对不同抗生素的检出限 (3σ)在 12 9～ 17 6ng·mL-1之间 ,其灵敏度的顺序是KANA >NEO >TOB >GEN .方法有较好的选择性 ,可用于市售抗生素注射液或滴耳液中药物含量和临床血药浓度的快速测定 .文中还用量子化学方法对反应机理进行探讨 。

In weak acid medium, some aminoglycoside antibiotics, such as kanamycin (KANA), gentamicin (GEN), tobramycin (TOB) and neomycin (NEO), or acid bisazo dye trypan red (TR) alone can only produce very weak resonance Rayleigh scattering (RRS) signals. However, when one of the antibiotics and TR react with each other to form the ion association complexes, the RRS intensity can be enhanced greatly and a new RRS spectrum and a significant enhancement of the RRS intensity in the wavelength range from 400 to 535 nm can be observed. The maximum scattering peak is at 400 nm and there is a linear relationship between the RRS intensity and the antibiotics concentration in the range of 0.013 similar to 6.0 mug (.) mL(-1). An RRS method for the determination of aminoglycoside antibiotics at trace amounts of levels has been developed. The detection limits (3sigma) of the four antibiotics, whose order of sensitivity from high to low ranks as KANA > NEO > TOB > GEN, are 12.9 similar to 17.6 ng (.) mL(-1). This method has good selectivity and has been successfully applied to the quick determination. of antibiotics not only for injections and ear drips, but also for clinic serum samples as well. In addition, the reaction mechanism by using quantum chemistry method and the influencing factors of the RRS spectra and the enhancement reasons of RRS have been discussed.