单分散交联聚甲基丙烯酸环氧丙酯树脂的制备及亲合纯化伴刀豆球蛋白-A

Monodisperse Porous Poly(glycidylmethacrylate-co-ethylene dimethacrylate) Particles as the Packings for High Performance Affinity Chromatography

采用一步种子溶胀聚合法制备了颗粒呈单分散的交联聚甲基丙烯酸环氧丙酯树脂 ,对其环氧基的组成比进行了表征 ,并以氨基葡萄糖为配基 ,首次制备了纯化粗品伴刀豆球蛋白 A (Con A ,Ⅲ )的聚合物基质的高效亲合色谱柱。配基在树脂上的键合量为 8.2mg g ,对纯化后的Con A 的吸附量为 13.4mg g。使用该亲合色谱介质成功地从粗品Con A中快速纯化了Con A ,电泳分析显示为一个主要的谱带 ,纯度从 15 %提高到 95 %。

Monodisperse poly(glycidylinethacrylate-co-ethylenedimethacrylate) (GMA-EDMA) particles were synthesized by one-step swelling and polymerization method. The concentration of the epoxide groups on the surface of beads was measured. The glucosamine was chosen as the ligand, and the packing materials for purification of concanavalin A (Con-A) were prepared. The capacity of the affinity matrix for Con-A was 13.4 mg/g. Con-A was purified from crude Con-A successfully for the first time by the affinity matrix. The purity of the purified Con-A was measured to be more than 95%.