纤维素-三(3,5-二甲基苯基氨基甲酸酯)涂敷氧化锆手性固定相的制备和色谱评价

Characterization of Derivatized Cellulose Coated Zirconia as Chiral Stationary Phase by High-Performance Liquid Chromatography

制备并表征了涂敷纤维素 三 (3,5 二甲基苯基氨基甲酸酯 ) ZrO2 手性固定相。以正己烷 /异丙醇为流动相 ,研究了正相色谱条件下手性固定相对几类光学异构体的手性拆分能力。实验结果表明 :氧化锆载体表面的碱性性质对光学对映体的手性拆分有很大影响 ;在常用流动相条件下 ,酸性对映体被完全滞留 ;纤维素 三 (3,5 二甲基苯基氨基甲酸酯 ) ZrO2 手性固定相对于中性及碱性对映异构体的分离呈现出较好的手性拆分能力。

The paper describes a procedure for preparing cellulose-tris (3, 5-dimethylphenylcarbamate) coated zirconia (CDMPC-coated ZrO2). Some neutral, basic and acidic enantiomers were enantioseparated under normal-phase conditions by high-performance liquid chromatography. Experimental results show that surface basic property of zirconia has profound influences on retention, selectivity and resolution of different racematic mixtures. Good enantiomeric resolutions for neutral and basic analytes were achieved, while acidic enantiomer did not elute from the column unless an acidic additive was presented in mobile phase.