摘要
菜心种子经^(60)Coγ射线辐照在离体培养中茎尖存活率随剂量增大而下降 ,茎尖增殖、子叶愈伤组织诱导和愈伤组织分化受到抑制 ;外植体龄影响离体培养中的辐射效应 ;小于 50Gy直接辐照离体培养中的茎尖促进芽的增殖。从对离体培养的效应看 ,对种子的适宜辐射剂量是200Gy左右 ,经催芽种子为 50～100Gy,离体培养茎尖可用 40～ 70Gy。
The survival rate in vitro of shoot tips of Brassica campestris L.var from seeds irradiated by 60 Co γ rays decreased with the increase of dose. Irradiation inhibited proliferation of shoot tip, induction of callus from cotyledons and differentiation of the callus. The age of explant contributed to the effect of irradiation in the culture. Irradiation stimulated the proliferation of shoot tip with dose less than 50Gy. Based on the effect of irradiation in the tissue culture, the effective dose recommended was about 200Gy for seeds , 50～100Gy for pre soaked germinating seeds and 40～70Gy for shoot tips in vitro , respectively.
出处
《核农学报》
CAS
CSCD
北大核心
2003年第4期264-268,共5页
Journal of Nuclear Agricultural Sciences
基金
广东省"九五"科技攻关项目 (970 30 8)
关键词
菜心
离体培养
辐射诱变
外植体龄
茎尖存活率
剂量
irradiation mutagensis
Brassica campestris L.var.
vegetable
tissue culture
