大鼠心肌缺血再灌注时能量代谢及脂质过氧化变化及复方丹参滴丸的保护作用(英文)

Energy metabolism and lipid peroxidation in rat myocardium with ischemia/reperfusion and protective effect of compound Danshen dripping pills

目的 探讨大鼠心肌缺血 再灌注时能量代谢及脂质过氧化变化及复方丹参滴丸的保护作用。方法 采用Langendorff离体心脏灌流技术 ,制备心肌缺血再灌注损伤模型 ,分别在缺血前预灌时及缺血后再灌注时用复方丹参滴丸对心肌给予保护 ,观察心肌能量代谢及脂质过氧化 (LPO)变化。本实验共分 4组 ,正常对照组 ,单纯缺血再灌注组 ,复方丹参滴丸前保护组 ,复方丹参滴丸后保护组。结果 ①心肌缺血 4 0min再灌注 2 0min ,心肌组织内高能磷酸化合物明显减少 ,脂质过氧化物含量明显增多 ,与正常对照组比P <0 .0 1。②在缺血前预灌注时及缺血后再灌注时加入复方丹参滴丸 ,心肌组织中的高能磷酸化合物均高于单纯缺血再灌注组 (P <0 .0 1) ,两组ATP、TAN均接近正常水平 (P >0 .0 5 )。两组的LPO低于单纯缺血再灌注组 ,复方丹参滴丸前保护组的LPO与对照组比无明显差别 (P >0 .0 5 )。结论 心肌缺血 4 0min再灌注 2 0min ,心肌能量代谢障碍 ,脂质过氧化反应增强 ,大鼠心肌发生缺血再灌注损伤。复方丹参滴丸在缺血前预灌注时与缺血后再灌注时给予均可通过增加心肌能量储备 ,抑制脂质过氧化物的生成而保护心肌。

Objective To study the energy metabolism and lipid peroxidation in rat myocardium with ischemia/reperfusion and the protective effect of compound Danshen dripping pill(DSDP).Methods The model of myocardium with ischemia/reperfusion was made in isolated rat heart perfused with Langendorff apparatus.Compound DSDP was given to myocardium in preperfusion before ischemia and in reperfusion after ischemia,to observe the changes of energy metabolism and lipid peroxide(LPO) in myocardium.There were four groups in the test,that is,control group,IR group,pre DS group and post DS group.Results ①In the time of 40 minute myocardial ischemia and 20 minute re perfusion,there was a remarkable decrease in high energy phoshphate and a distinct increase in lipid peroxide in myocardium,compared with those in control group( P <0.01).②When DSDP was given in preperfusion before and after ischemia,high energy phosphate was higher than that in only ischemia/reperfusion group( P <0.01).In both groups,ATP and total adenosine pool (TAN) were close to normal( P >0.05),and LPO was lower than that in IR group( P <0.01).LPO in pre DS group was not significantly different from that in control group( P >0.05).Conclusion When myocardium was ischemined for 40min then reperfused for 20min,high energy phosphate synthesis decreased and lipid peroxidation increased,and myocardium suffered from ischemia/reperfusion injury.Either given in pre perfusion before ischemia and post perfusion after ischemia,DSDP could increase the energy storage of myocardium and protect it by inhibiting the generation of lipid peroxide.So DSDP could be used in prevention and treatment of ischemia reperfusion injury.